Different intracellular and intranuclear transport of triiodothyronine enantiomers in rat skeletal myoblasts

Alfredo Pontecorvi, Mark Lakshmanan, Jacob Robbins

Risultato della ricerca: Contributo in rivistaArticolo in rivista

11 Citazioni (Scopus)

Abstract

The mechanism(s) responsible for the different biological potency of L- and D-T3 was investigated in rat L6E9 myoblasts. After incubation with intact cells at 37 C L-T3 cellular and nuclear uptakes were 91% and 70% higher than those of D-T3, respectively, but values for nuclear uptake as a fraction of cellular uptake were similar. The difference between the enantiomers was abolished at 4 C, and metabolic and endocytotic inhibitors reduced nuclear and extranuclear saturable uptake of L-T3 to a similar degree, but had little or no effect on D-T3 uptake. The affinity constants (Ka) for L- and D-T3 binding to isolated nuclei were similar, but the apparent nuclear Ka of L-T3 in intact cells was 5-fold greater than that of D-T3. The findings suggest that stereospecific transport, mainly active at the plasma membrane, occurs in rat skeletal muscle cells. This discriminative pathway of cell entry facilitates L-T3 uptake by an energy-dependent pathway not shared by D-T3 and may explain the greater potency of L-T3 than D-T3.
Lingua originaleEnglish
pagine (da-a)2922-2929
Numero di pagine8
RivistaEndocrinology
Volume123
Stato di pubblicazionePubblicato - 1988

Keywords

  • Animals
  • Antimycin A
  • Biological Transport
  • Cadaverine
  • Cell Line
  • Cell Membrane
  • Cell Nucleus
  • Iodine Radioisotopes
  • Kinetics
  • Muscles
  • Oligomycins
  • Rats
  • Stereoisomerism
  • Triiodothyronine

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