TY - JOUR
T1 - Different effects of matrix degrading enzymes towards biofilms formed by E. faecalis and E. faecium clinical isolates
AU - Torelli, Riccardo
AU - Cacaci, Margherita
AU - Papi, Massimiliano
AU - Paroni Sterbini, Francesco
AU - Martini, Cecilia
AU - Posteraro, Brunella
AU - Palmieri, Valentina
AU - De Spirito, Marco
AU - Sanguinetti, Maurizio
AU - Bugli, Francesca
PY - 2017
Y1 - 2017
N2 - E. faecalis and E. faecium cause urinary tract infections highly resistant to therapies due to a protective extracellular matrix. To exploit a new strategy able to treat infections without increasing antibiotic doses, we used enzymes targeting specific biofilm matrix components in combination with Vancomycin. We investigated the activity of Vancomycin combined with two matrix-degrading enzymes, Alginate Lyase (AlgL) and Deoxyribonuclease I (DNase I) against in vitro biofilm of E. faecalis and E. faecium clinical isolates. The heterogeneity of matrix composition leads to defined physiological responses of biofilm communities to their environment: we demonstrated that the use of DNase I and AlgL enzymes affects biofilm structure, cell viability and reduces MBEC values of Vancomycin in E. faecalis and E. faecium, respectively.
AB - E. faecalis and E. faecium cause urinary tract infections highly resistant to therapies due to a protective extracellular matrix. To exploit a new strategy able to treat infections without increasing antibiotic doses, we used enzymes targeting specific biofilm matrix components in combination with Vancomycin. We investigated the activity of Vancomycin combined with two matrix-degrading enzymes, Alginate Lyase (AlgL) and Deoxyribonuclease I (DNase I) against in vitro biofilm of E. faecalis and E. faecium clinical isolates. The heterogeneity of matrix composition leads to defined physiological responses of biofilm communities to their environment: we demonstrated that the use of DNase I and AlgL enzymes affects biofilm structure, cell viability and reduces MBEC values of Vancomycin in E. faecalis and E. faecium, respectively.
KW - Alginate lyase
KW - Biofilm
KW - Biotechnology
KW - Colloid and Surface Chemistry
KW - Deoxyribonuclease I
KW - E. faecalis
KW - E. faecium
KW - Physical and Theoretical Chemistry
KW - Surfaces and Interfaces
KW - Vancomycin
KW - Alginate lyase
KW - Biofilm
KW - Biotechnology
KW - Colloid and Surface Chemistry
KW - Deoxyribonuclease I
KW - E. faecalis
KW - E. faecium
KW - Physical and Theoretical Chemistry
KW - Surfaces and Interfaces
KW - Vancomycin
UR - https://publicatt.unicatt.it/handle/10807/109765
UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85023597685&origin=inward
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85023597685&origin=inward
U2 - 10.1016/j.colsurfb.2017.07.010
DO - 10.1016/j.colsurfb.2017.07.010
M3 - Article
SN - 0927-7765
VL - 158
SP - 349
EP - 355
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
IS - ottobre
ER -