Different effects of matrix degrading enzymes towards biofilms formed by E. faecalis and E. faecium clinical isolates

Massimiliano Papi, Brunella Posteraro, Marco De Spirito, Maurizio Sanguinetti, Francesca Bugli, Riccardo Torelli, Margherita Cacaci, Francesco Paroni Sterbini, Cecilia Martini, Valentina Palmieri

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

11 Citazioni (Scopus)

Abstract

E. faecalis and E. faecium cause urinary tract infections highly resistant to therapies due to a protective extracellular matrix. To exploit a new strategy able to treat infections without increasing antibiotic doses, we used enzymes targeting specific biofilm matrix components in combination with Vancomycin. We investigated the activity of Vancomycin combined with two matrix-degrading enzymes, Alginate Lyase (AlgL) and Deoxyribonuclease I (DNase I) against in vitro biofilm of E. faecalis and E. faecium clinical isolates. The heterogeneity of matrix composition leads to defined physiological responses of biofilm communities to their environment: we demonstrated that the use of DNase I and AlgL enzymes affects biofilm structure, cell viability and reduces MBEC values of Vancomycin in E. faecalis and E. faecium, respectively.
Lingua originaleEnglish
pagine (da-a)349-355
Numero di pagine7
RivistaCOLLOIDS AND SURFACES. B, BIOINTERFACES
Volume158
DOI
Stato di pubblicazionePubblicato - 2017

Keywords

  • Alginate lyase
  • Biofilm
  • Biotechnology
  • Colloid and Surface Chemistry
  • Deoxyribonuclease I
  • E. faecalis
  • E. faecium
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces
  • Vancomycin

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