Different effects of matrix degrading enzymes towards biofilms formed by E. faecalis and E. faecium clinical isolates

Riccardo Torelli; Margherita Cacaci; Massimiliano Papi; Francesco Paroni Sterbini; Cecilia Martini; Brunella Posteraro; Valentina Palmieri; Marco De Spirito; Maurizio Sanguinetti; Francesca Bugli

doi:10.1016/j.colsurfb.2017.07.010

Different effects of matrix degrading enzymes towards biofilms formed by E. faecalis and E. faecium clinical isolates

Riccardo Torelli, Margherita Cacaci, Massimiliano Papi^*, Francesco Paroni Sterbini, Cecilia Martini, Brunella Posteraro, Valentina Palmieri, Marco De Spirito, Maurizio Sanguinetti, Francesca Bugli

^*Autore corrispondente per questo lavoro

Risultato della ricerca: Contributo in rivista › Articolo in rivista › peer review

13 Citazioni (Scopus)

Abstract

E. faecalis and E. faecium cause urinary tract infections highly resistant to therapies due to a protective extracellular matrix. To exploit a new strategy able to treat infections without increasing antibiotic doses, we used enzymes targeting specific biofilm matrix components in combination with Vancomycin. We investigated the activity of Vancomycin combined with two matrix-degrading enzymes, Alginate Lyase (AlgL) and Deoxyribonuclease I (DNase I) against in vitro biofilm of E. faecalis and E. faecium clinical isolates. The heterogeneity of matrix composition leads to defined physiological responses of biofilm communities to their environment: we demonstrated that the use of DNase I and AlgL enzymes affects biofilm structure, cell viability and reduces MBEC values of Vancomycin in E. faecalis and E. faecium, respectively.

Lingua originale	English
pagine (da-a)	349-355
Numero di pagine	7
Rivista	COLLOIDS AND SURFACES. B, BIOINTERFACES
Volume	158
DOI	https://doi.org/10.1016/j.colsurfb.2017.07.010
Stato di pubblicazione	Pubblicato - 2017

Keywords

Alginate lyase
Biofilm
Biotechnology
Colloid and Surface Chemistry
Deoxyribonuclease I
E. faecalis
E. faecium
Physical and Theoretical Chemistry
Surfaces and Interfaces
Vancomycin

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10.1016/j.colsurfb.2017.07.010

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Torelli, R., Cacaci, M., Papi, M., Paroni Sterbini, F., Martini, C., Posteraro, B., Palmieri, V., De Spirito, M., Sanguinetti, M., & Bugli, F. (2017). Different effects of matrix degrading enzymes towards biofilms formed by E. faecalis and E. faecium clinical isolates. COLLOIDS AND SURFACES. B, BIOINTERFACES, 158, 349-355. https://doi.org/10.1016/j.colsurfb.2017.07.010

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abstract = "E. faecalis and E. faecium cause urinary tract infections highly resistant to therapies due to a protective extracellular matrix. To exploit a new strategy able to treat infections without increasing antibiotic doses, we used enzymes targeting specific biofilm matrix components in combination with Vancomycin. We investigated the activity of Vancomycin combined with two matrix-degrading enzymes, Alginate Lyase (AlgL) and Deoxyribonuclease I (DNase I) against in vitro biofilm of E. faecalis and E. faecium clinical isolates. The heterogeneity of matrix composition leads to defined physiological responses of biofilm communities to their environment: we demonstrated that the use of DNase I and AlgL enzymes affects biofilm structure, cell viability and reduces MBEC values of Vancomycin in E. faecalis and E. faecium, respectively.",

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author = "Riccardo Torelli and Margherita Cacaci and Massimiliano Papi and {Paroni Sterbini}, Francesco and Cecilia Martini and Brunella Posteraro and Valentina Palmieri and {De Spirito}, Marco and Maurizio Sanguinetti and Francesca Bugli",

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Torelli, R , Cacaci, M , Papi, M, Paroni Sterbini, F, Martini, C, Posteraro, B, Palmieri, V, De Spirito, M , Sanguinetti, M & Bugli, F 2017, 'Different effects of matrix degrading enzymes towards biofilms formed by E. faecalis and E. faecium clinical isolates', COLLOIDS AND SURFACES. B, BIOINTERFACES, vol. 158, pagg. 349-355. https://doi.org/10.1016/j.colsurfb.2017.07.010

TY - JOUR

T1 - Different effects of matrix degrading enzymes towards biofilms formed by E. faecalis and E. faecium clinical isolates

AU - Torelli, Riccardo

AU - Cacaci, Margherita

AU - Papi, Massimiliano

AU - Paroni Sterbini, Francesco

AU - Martini, Cecilia

AU - Posteraro, Brunella

AU - Palmieri, Valentina

AU - De Spirito, Marco

AU - Sanguinetti, Maurizio

AU - Bugli, Francesca

PY - 2017

Y1 - 2017

N2 - E. faecalis and E. faecium cause urinary tract infections highly resistant to therapies due to a protective extracellular matrix. To exploit a new strategy able to treat infections without increasing antibiotic doses, we used enzymes targeting specific biofilm matrix components in combination with Vancomycin. We investigated the activity of Vancomycin combined with two matrix-degrading enzymes, Alginate Lyase (AlgL) and Deoxyribonuclease I (DNase I) against in vitro biofilm of E. faecalis and E. faecium clinical isolates. The heterogeneity of matrix composition leads to defined physiological responses of biofilm communities to their environment: we demonstrated that the use of DNase I and AlgL enzymes affects biofilm structure, cell viability and reduces MBEC values of Vancomycin in E. faecalis and E. faecium, respectively.

AB - E. faecalis and E. faecium cause urinary tract infections highly resistant to therapies due to a protective extracellular matrix. To exploit a new strategy able to treat infections without increasing antibiotic doses, we used enzymes targeting specific biofilm matrix components in combination with Vancomycin. We investigated the activity of Vancomycin combined with two matrix-degrading enzymes, Alginate Lyase (AlgL) and Deoxyribonuclease I (DNase I) against in vitro biofilm of E. faecalis and E. faecium clinical isolates. The heterogeneity of matrix composition leads to defined physiological responses of biofilm communities to their environment: we demonstrated that the use of DNase I and AlgL enzymes affects biofilm structure, cell viability and reduces MBEC values of Vancomycin in E. faecalis and E. faecium, respectively.

KW - Alginate lyase

KW - Biofilm

KW - Biotechnology

KW - Colloid and Surface Chemistry

KW - Deoxyribonuclease I

KW - E. faecalis

KW - E. faecium

KW - Physical and Theoretical Chemistry

KW - Surfaces and Interfaces

KW - Vancomycin

KW - Alginate lyase

KW - Biofilm

KW - Biotechnology

KW - Colloid and Surface Chemistry

KW - Deoxyribonuclease I

KW - E. faecalis

KW - E. faecium

KW - Physical and Theoretical Chemistry

KW - Surfaces and Interfaces

KW - Vancomycin

UR - http://hdl.handle.net/10807/109765

UR - http://www.elsevier.com/locate/colsurfb

U2 - 10.1016/j.colsurfb.2017.07.010

DO - 10.1016/j.colsurfb.2017.07.010

M3 - Article

SN - 0927-7765

VL - 158

SP - 349

EP - 355

JO - COLLOIDS AND SURFACES. B, BIOINTERFACES

JF - COLLOIDS AND SURFACES. B, BIOINTERFACES

ER -

Different effects of matrix degrading enzymes towards biofilms formed by E. faecalis and E. faecium clinical isolates

Abstract

Keywords

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