TY - JOUR
T1 - Dietary supplementation with eicosapentaenoic and docosahexaenoic acid inhibits growth of Morris hepatocarcinoma 3924A in rats: effects on proliferation and apoptosis.
AU - Calviello, Gabriella
AU - Palozza, Paola
AU - Piccioni, Elisabetta
AU - Maggiano, Nicola
AU - Frattucci, Andrea
AU - Franceschelli, Piergiorgio
AU - Bartoli, Gianna
AU - Maria,
PY - 1998
Y1 - 1998
N2 - Int J Cancer. 1998 Mar 2;75(5):699-705.
Dietary supplementation with eicosapentaenoic and docosahexaenoic acid inhibits
growth of Morris hepatocarcinoma 3924A in rats: effects on proliferation and
apoptosis.
Calviello G, Palozza P, Piccioni E, Maggiano N, Frattucci A, Franceschelli P,
Bartoli GM.
Institute of General Pathology, Catholic University, Rome, Italy.
The effect of individual administration of low doses of highly purified
eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) (1 g/kg body weight)
on the growth of Morris hepatocarcinoma 3924A transplanted in ACI/T rats was
investigated. Both EPA and DHA inhibited growth of the hepatocarcinoma (50%
reduction of tumor weight or volume at the 19th day after transplantation for
both of the n-3 PUFA groups). EPA treatment reduced the percentage of
proliferating tumor cells labeled with BUdR (10-fold), whereas DHA did not.
Conversely, DHA supplementation induced a doubling of the number of cells
undergoing apoptosis (labeled by TUNEL), whereas EPA treatment was much less
effective. Analysis of changes in phospholipid fatty acids in tumor-cell
membranes after both treatments with EPA and DHA showed a significant reduction
in arachidonic-acid levels. EPA and docosapentaenoic acid (DPA), its elongation
product, were increased in the phospholipids from EPA-treated animals. DHA and
EPA, but not DPA, were increased in the DHA-treated group. It is concluded from
the results of the present study that the anti-tumoral effect of EPA is related
mainly to its inhibition of cell proliferation, whereas that of DHA corresponds
with its induction of apoptosis. The alterations in fatty-acid composition
induced by EPA or DHA appear to be factors underlying their differential actions
on cell proliferation and apoptosis.
AB - Int J Cancer. 1998 Mar 2;75(5):699-705.
Dietary supplementation with eicosapentaenoic and docosahexaenoic acid inhibits
growth of Morris hepatocarcinoma 3924A in rats: effects on proliferation and
apoptosis.
Calviello G, Palozza P, Piccioni E, Maggiano N, Frattucci A, Franceschelli P,
Bartoli GM.
Institute of General Pathology, Catholic University, Rome, Italy.
The effect of individual administration of low doses of highly purified
eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) (1 g/kg body weight)
on the growth of Morris hepatocarcinoma 3924A transplanted in ACI/T rats was
investigated. Both EPA and DHA inhibited growth of the hepatocarcinoma (50%
reduction of tumor weight or volume at the 19th day after transplantation for
both of the n-3 PUFA groups). EPA treatment reduced the percentage of
proliferating tumor cells labeled with BUdR (10-fold), whereas DHA did not.
Conversely, DHA supplementation induced a doubling of the number of cells
undergoing apoptosis (labeled by TUNEL), whereas EPA treatment was much less
effective. Analysis of changes in phospholipid fatty acids in tumor-cell
membranes after both treatments with EPA and DHA showed a significant reduction
in arachidonic-acid levels. EPA and docosapentaenoic acid (DPA), its elongation
product, were increased in the phospholipids from EPA-treated animals. DHA and
EPA, but not DPA, were increased in the DHA-treated group. It is concluded from
the results of the present study that the anti-tumoral effect of EPA is related
mainly to its inhibition of cell proliferation, whereas that of DHA corresponds
with its induction of apoptosis. The alterations in fatty-acid composition
induced by EPA or DHA appear to be factors underlying their differential actions
on cell proliferation and apoptosis.
KW - Docosahexaenoic acid
KW - Eicosapentaenoic acid
KW - Morris hepatoma
KW - apoptosis
KW - proliferation
KW - rats
KW - Docosahexaenoic acid
KW - Eicosapentaenoic acid
KW - Morris hepatoma
KW - apoptosis
KW - proliferation
KW - rats
UR - http://hdl.handle.net/10807/6707
M3 - Article
SN - 0020-7136
SP - 699
EP - 705
JO - International Journal of Cancer
JF - International Journal of Cancer
ER -