Diacylglycerol kinase-alpha phosphorylation by Src on Y335 is required for activation, membrane recruitment and Hgf-induced cell motility

Ornella Parolini, G. Baldanzi, S. Cutrupi, F. Chianale, V. Gnocchi, E. Rainero, P. Porporato, N. Filigheddu, W. J. Van Blitterswijk, F. Bussolino, F. Sinigaglia, A. Graziani

Risultato della ricerca: Contributo in rivistaArticolo in rivista

35 Citazioni (Scopus)

Abstract

Diacylglycerol (DAG) kinases (Dgk), which phosphorylate DAG to generate phosphatidic acid, act as either positive or negative key regulators of cell signaling. We previously showed that Src mediates growth factors-induced activation of Dgk-alpha, whose activity is required for cell motility, proliferation and angiogenesis. Here, we demonstrate that both hepatocytes growth factor (HGF) stimulation and v-Src transformation induce tyrosine phosphorylation of Dgk-alpha on Y335, through a mechanism requiring its proline-rich C-terminal sequence. Moreover, we show that both proline-rich sequence and phosphorylation of Y335 of Dgk-alpha mediate: (i) its enzymatic activation, (ii) its ability to interact respectively with SH3 and SH2 domains of Src, (iii) its recruitment to the membrane. In addition, we show that phosphorylation of Dgk-alpha on Y335 is required for HGF-induced motility, while its constitutive recruitment at the membrane by myristylation is sufficient to trigger spontaneous motility in absence of HGF. Providing the first evidence that tyrosine phosphorylation of Dgk-alpha is required for growth-factors-induced activation and membrane recruitment, these findings underscore its relevance as a rheostat, whose activation is a threshold to elicit growth factors-induced migratory signaling.
Lingua originaleEnglish
pagine (da-a)942-956
Numero di pagine15
RivistaOncogene
Volume27
DOI
Stato di pubblicazionePubblicato - 2008

Keywords

  • Animals
  • COS Cells
  • Cell Communication
  • Cell Membrane
  • Cell Movement
  • Cells, Cultured
  • Cercopithecus aethiops
  • Diacylglycerol Kinase
  • Dogs
  • Enzyme Activation
  • Hepatocyte Growth Factor
  • Humans
  • Kidney
  • Myristic Acids
  • Oncogene Protein pp60(v-src)
  • Phosphorylation
  • Proline
  • Signal Transduction
  • Tyrosine

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