Development of an uplc-ms/ms method for quantitative analysis of clotrimazole in human plasma samples

Risultato della ricerca: Contributo in rivistaArticolo in rivista

Abstract

An ultra-performance liquid chromatography-tandem mass spectrometry method was developed for the quantification of clotrimazole (CTZ) plasma levels after intravaginal administration of the drug given at approved dosages. Plasma samples were extracted by liquid–liquid extraction and a single chromatographic run could be completed within about 2 min. The method was linear over the investigated range (0.488–250 ng/mL) with all the correlation coefficients, R2, greater than 0.9903. All data were in the range of ±15.0% with respect to the nominal concentration for high QC and medium QC, and in the range ±20% with respect to the nominal concentration for low QC. This rapid and sensitive method was validated and could be applied to human plasma samples from a healthy volunteer, showing that the assay is able to detect plasma concentrations of CTZ in the range of those found after the administration of the drug at approved dosages in the clinical setting.
Lingua originaleEnglish
pagine (da-a)1-9
Numero di pagine9
RivistaSeparations
Volume7
DOI
Stato di pubblicazionePubblicato - 2020

Keywords

  • Bioequivalence
  • Clotrimazole
  • UPLC/MS-MS
  • Plasma
  • Intravaginal treatment

Fingerprint Entra nei temi di ricerca di 'Development of an uplc-ms/ms method for quantitative analysis of clotrimazole in human plasma samples'. Insieme formano una fingerprint unica.

Cita questo