Development of a headspace solid-phase microextraction gas chromatography-mass spectrometric method for the determination of short-chain fatty acids from intestinal fermentation

Margherita Dall'Asta, F. Bianchi, D. Del Rio, A. Mangia, M. Musci, F. Scazzina

Risultato della ricerca: Contributo in rivistaArticolo in rivista

44 Citazioni (Scopus)

Abstract

The determination of short-chain fatty acids produced by colonic fermentation can provide useful information as a tool to evaluate prebiotic effects of foods with beneficial effects on human health. In this study, we developed and validated a headspace solid-phase microextraction gas chromatographic-mass spectrometric method for the determination of underivatised short-chain fatty acids produced in an in vitro fermentation model. A divinylbenzene/Carboxen/polydimethylsiloxane fibre was used. Solid-phase microextraction conditions, i.e., extraction temperature, extraction time and salt addition were optimised by means of a central composite face-centred experimental design. The method was validated in terms of limit of quantification, accuracy, precision and linearity. The developed method was then applied to the analysis of short-chain fatty acids produced by the fermentation of faecal cultures derived from bread enriched with different kinds of dietary fibres. © 2011 Elsevier Ltd. All rights reserved.
Lingua originaleEnglish
pagine (da-a)200-205
Numero di pagine6
RivistaFood Chemistry
Volume129
DOI
Stato di pubblicazionePubblicato - 2011

Keywords

  • Faecal cultures
  • Fermentation
  • Short-chain fatty acids
  • Solid-phase microextraction

Fingerprint Entra nei temi di ricerca di 'Development of a headspace solid-phase microextraction gas chromatography-mass spectrometric method for the determination of short-chain fatty acids from intestinal fermentation'. Insieme formano una fingerprint unica.

Cita questo