Abstract
Background: Asymmetric dimethylarginine (ADMA), an endogenous
competitive inhibitor of nitric oxide synthase plays an important role
in endothelial dysfunction processes. Recent studies have linked high
ADMA levels with several pathological conditions. The interest as a
marker of endothelial dysfunction has increased in the last few years.
In this paper, a method for serum ADMA quantification by liquid
chromatography tandem mass spectrometry has been described. To test the
utility in a pathological condition ADMA levels in hypertensive subjects
have been measured.
Methods: HPLC separation was performed by hydrophilic interaction
chromatography using acetonitrile/water containing 0.1\% formic acid and
20 mmol/L ammonium formate. Selected reaction monitoring was performed
following the transitions m/z 203.1 -> 46.4 for ADMA and 210.1 -> 46.3
for the internal standard {[H-2(7)]ADMA.
Results: The method was linear up to 10 mu mol/L, limit of detection and
limit of quantification were 0.005 mu mol/L and 0.01 mu mol/L,
respectively. Recovery was higher than 96\%. Intra- and inter-assay
imprecision were lower than 6\%. The accuracy, expressed as bias \%, was
<2.5. ADMA in ``healthy'' subjects ranged from 0.343 to 0.608 mu mol/L
and resulted significantly lower than that measured in hypertensive
subjects (p < 0.001).
Conclusions: The method developed is selective and sensitive, thus
suitable not only for research purposes, but also for routinely work.}
Lingua originale | English |
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pagine (da-a) | 2109-2115 |
Numero di pagine | 7 |
Rivista | Clinical Chemistry and Laboratory Medicine |
Volume | 49 |
DOI | |
Stato di pubblicazione | Pubblicato - 2011 |
Keywords
- ADMA, LC-MS/MS