Detection of tumor-derived DNA in cerebrospinal fluid of patients with primary tumors of the brain and spinal cord

Alessandro Olivi, Yuxuan Wang, Simeon Springer, Ming Zhang, K. Wyatt Mcmahon, Isaac Kinde, Lisa Dobbyn, Janine Ptak, Henry Brem, Kaisorn Chaichana, Gary L. Gallia, Ziya L. Gokaslan, Mari L. Groves, George I. Jallo, Michael Lim, Alfredo Quinones-Hinojosa, Daniele Rigamonti, Greg J. Riggins, Daniel M. Sciubba, Jon D. WeingartJean-Paul Wolinsky, Xiaobu Ye, Sueli Mieko Oba-Shinjo, Suely K. N. Marie, Matthias Holdhoff, Nishant Agrawal, Luis A. Diaz, Nickolas Papadopoulos, Kenneth W. Kinzler, Bert Vogelstein, Chetan Bettegowda

Risultato della ricerca: Contributo in rivistaArticolo in rivista

163 Citazioni (Scopus)

Abstract

Cell-free DNA shed by cancer cells has been shown to be a rich source of putative tumor-specific biomarkers. Because cell-free DNA from brain and spinal cord tumors cannot usually be detected in the blood, we studied whether the cerebrospinal fluid (CSF) that bathes the CNS is enriched for tumor DNA, here termed CSF-tDNA. We analyzed 35 primary CNS malignancies and found at least one mutation in each tumor using targeted or genome-wide sequencing. Using these patient-specific mutations as biomarkers, we identified detectable levels of CSF-tDNA in 74% [95% confidence interval (95% CI) = 57-88%] of cases. All medulloblastomas, ependymomas, and high-grade gliomas that abutted a CSF space were detectable (100% of 21 cases; 95% CI = 88-100%), whereas no CSF-tDNA was detected in patients whose tumors were not directly adjacent to a CSF reservoir (P < 0.0001, Fisher's exact test). These results suggest that CSF-tDNA could be useful for the management of patients with primary tumors of the brain or spinal cord.
Lingua originaleEnglish
pagine (da-a)9704-9709
Numero di pagine6
RivistaProceedings of the National Academy of Sciences of the United States of America
Volume112
DOI
Stato di pubblicazionePubblicato - 2015

Keywords

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarker
  • Brain Neoplasms
  • CNS tumors
  • CSF-tDNA
  • Child
  • Child, Preschool
  • DNA Mutational Analysis
  • DNA, Neoplasm
  • Demography
  • Exons
  • Female
  • Genome, Human
  • Humans
  • Magnetic Resonance Imaging
  • Male
  • Middle Aged
  • Multivariate Analysis
  • Mutation
  • Spinal Cord Neoplasms

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