Description of an Automated Method for Urea Nitrogen Determination in Bronchoalveolar Lavage Fluid (BALF) of Neonates and Infants

Giorgio Conti, Krizia Pocino, Angelo Minucci, Rocco Manieri, Daniele De Luca, Ettore Domenico Capoluongo

Risultato della ricerca: Contributo in rivistaArticolo in rivista

7 Citazioni (Scopus)

Abstract

Bronchoalveolar lavage (BAL) partially recovers both the instilled saline and the alveolar fluid, so-called epithelial lining fluid (ELF), but a correction for the dilution due to the BAL technique itself is needed to know the amount of recovered ELF. In this regard, urea nitrogen may be useful and has been proposed to calculate ELF. The aim of the present study was to develop and validate a new method to measure urea nitrogen in BAL fluid (BALF). We used 19 BALF samples obtained from neonates and infants with different respiratory conditions. The urea nitrogen assay was carried out on Cobas c311 analyzer (Roche Diagnostics). A validation study shows that the method is perfectly linear (R-2 = 0.999), sensitive (limit of detection = 0.055 mg/dL; limit of quantification = 0.16 mg/dL), repeatable (low = 0.15 +/- 0.02, 13.3%; high = 1.80 +/- 0.02, 1.1%), reproducible (low = 0.14 +/- 0.02, 14.2 %; high = 1.76 +/- 0.04, 2.2 %) with accuracy ranging between 93-96%. Our results support the robustness of validated procedure since the described method appears simple, precise, rapid, and suitable for routine analysis. Thus, it may be used to correct concentration of various noncellular BAL components and calculate their ELF amounts in neonates and infants.
Lingua originaleEnglish
pagine (da-a)636-641
Numero di pagine6
RivistaJALA
Volume20
DOI
Stato di pubblicazionePubblicato - 2015

Keywords

  • method validation
  • urea nitrogen assay

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