TY - JOUR
T1 - Cytoplasmic expression of oestrogen receptor beta (ERβ) as a prognostic factor in vulvar squamous cell carcinoma in elderly women.
AU - Scambia, Giovanni
AU - Zannoni, Gian Franco
AU - Prisco, Mg
AU - Vellone, Valerio Gaetano
AU - De Stefano, Ilaria
AU - Vizzielli, Giuseppe
AU - Tortorella, Lucia
AU - Fagotti, Anna
AU - Gallo Guido, Daniela
PY - 2011
Y1 - 2011
N2 - AIMS: To investigate the prognostic value of cytoplasmic oestrogen receptor beta (ERβ) expression in a series of untreated patients with non-human papillomavirus (HPV)-related vulvar cancer.
METHODS AND RESULTS: Immunohistochemistry was carried out using a polyclonal rabbit anti-human ERβ antibody. The nuclear and cytoplasmic expression of ERβ was evaluated in 33 patients. Cytoplasmic immunoreactivity was correlated with histopathological and molecular parameters (Ki67, p21), disease-free survival (DFS) and overall survival (OS). The expression of cytoplasmic ERβ was found to be associated with grade (P=0.006), while no association was found with any of the remaining variables examined. Cases with high cytoplasmic ERβ expression showed lower DFS and OS compared to cases with low cytoplasmic ERβ (P=0.007, P=0.01, respectively). There was also a progressive decline in both the DFS and OS with increasing tumour size (P=0.05, P=0.07, respectively) and with increasing depth of infiltration (P=0.14, P=0.07, respectively). On multivariate analysis, only tumour size and cytoplasmic ERβ staining retained an independent negative prognostic role for DFS and OS.
CONCLUSIONS: The assessment of cytoplasmic ERβ expression could be helpful to identify poor prognosis in elderly patients with non-HPV-related vulvar squamous cell carcinoma (SCC).
AB - AIMS: To investigate the prognostic value of cytoplasmic oestrogen receptor beta (ERβ) expression in a series of untreated patients with non-human papillomavirus (HPV)-related vulvar cancer.
METHODS AND RESULTS: Immunohistochemistry was carried out using a polyclonal rabbit anti-human ERβ antibody. The nuclear and cytoplasmic expression of ERβ was evaluated in 33 patients. Cytoplasmic immunoreactivity was correlated with histopathological and molecular parameters (Ki67, p21), disease-free survival (DFS) and overall survival (OS). The expression of cytoplasmic ERβ was found to be associated with grade (P=0.006), while no association was found with any of the remaining variables examined. Cases with high cytoplasmic ERβ expression showed lower DFS and OS compared to cases with low cytoplasmic ERβ (P=0.007, P=0.01, respectively). There was also a progressive decline in both the DFS and OS with increasing tumour size (P=0.05, P=0.07, respectively) and with increasing depth of infiltration (P=0.14, P=0.07, respectively). On multivariate analysis, only tumour size and cytoplasmic ERβ staining retained an independent negative prognostic role for DFS and OS.
CONCLUSIONS: The assessment of cytoplasmic ERβ expression could be helpful to identify poor prognosis in elderly patients with non-HPV-related vulvar squamous cell carcinoma (SCC).
KW - vulvar squamous cell carcinoma
KW - vulvar squamous cell carcinoma
UR - http://hdl.handle.net/10807/3755
M3 - Article
SN - 0309-0167
VL - 2011
SP - 909
EP - 917
JO - Histopathology
JF - Histopathology
ER -