Cytogenetic characterization of the holocentric chromosomes in the aphids Myzus varians and Myzus cerasi

Emanuele Mazzoni, D. Bizzaro, E. Barbolini, M. Mandrioli, G. C. Manicardi

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18 Citazioni (Scopus)

Abstract

Cytogenetic investigations on the holocentric chromosomes of Myzus varians and Myzus cerasi have been carried out using silver staining and C-banding followed by chromomycin A3 (CMA3) and DAPI staining in order to improve our knowledge of these pest crop species and facilitate their identification. In M. varians C-banding pattern is pecu-: liar of each chromosome pair thus allowing the identification of all homologues and the reconstruction of a reliable karyotype whereas in M. cerasi, C-positive regions result to be limited to both the telomeric regions of the X chromosomes. In both species, silver staining localizes rDNA genes on one telomere of each X chromosome; such telomeres are the unique brightly fluorescent C-positive regions after CMA3 staining, whereas all other heterochromatic bands result DAPI positive.
Lingua originaleEnglish
pagine (da-a)81-85
Numero di pagine5
RivistaCaryologia
Volume52
Stato di pubblicazionePubblicato - 1999

Keywords

  • Aphididae
  • Cytogenetic
  • Heterochromatin
  • Holocentric chromosomes

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