Cross talk between cancer and immune cells: exploring complex dynamics in a microfluidic environment

Gabriele Ciasca, Luca Businaro, Adele De Ninno, Giovanna Schiavoni, Valeria Lucarini, Annamaria Gerardino, Filippo Belardelli, Lucia Gabriele, Fabrizio Mattei

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

82 Citazioni (Scopus)

Abstract

The reconstitution of a complex microenvironment on microfluidic chips is one of the cornerstones to demonstrate the improved flexibility of these devices with respect to macroscale in vitro approaches. In this work, we realised an on-chip model to investigate the interactions between cancer and immune system. To this end, we exploited mice deficient (Knock Out, KO) for interferon regulatory factor 8 (IRF-8), a transcription factor essential for the induction of competent immune responses, to investigate how IRF-8 gene expression contributes to regulate immune and melanoma cells crosstalk. In vivo, IRF-8 KO mice are highly permissive to B16 melanoma growth due to failure of immune cells to properly exert immunosurveillance. B16 cells and immune cells isolated from the spleen of wild type (WT) and IRF-8 KO mice were co-cultured for one week in a PDMS platform and monitored by fluorescence microscopy and time-lapse recordings. We observed that WT spleen cells migrated through microchannels connecting the culturing chambers towards B16 cells and tightly interacted with tumor cells, forming clusters of activation. In contrast, IRF-8 KO immune cells poorly interacted with melanoma cells. In parallel, B16 cells were more attracted towards microchannels, acquiring a more invasive behaviour in the presence of IRF-8 KO spleen cells, with respect to WT cells. Our results strongly confirm the in vivo observations and highlight the value of on-chip co-culture systems as a useful in vitro tool to elucidate the reciprocal interactions between cancer cells and host immune system, with relevant impact in the development of more effective anti-tumor therapeutic strategies.
Lingua originaleEnglish
pagine (da-a)229-239
Numero di pagine11
RivistaLAB ON A CHIP
Volume13
DOI
Stato di pubblicazionePubblicato - 2013

Keywords

  • cancer, immune cells, Lab on Chip

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