Complex landscape of protein structural dynamics unveiled by nanosecond Laue crystallography

Alessandro Arcovito, Dominique Bourgeois, Beatrice Vallone, Friederich Schotte, Adriana Erica Miele, Giuliano Sciara, Micheal Wulff, Philip Anfinrud, Maurizio Brunori

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

179 Citazioni (Scopus)

Abstract

Although conformational changes are essential for the function of proteins, little is known about their structural dynamics at atomic level resolution. Myoglobin (Mb) is the paradigm to investigate conformational dynamics because it is a simple globular heme protein displaying a photosensitivity of the iron-ligand bond. Upon laser photodissociation of carboxymyoglobin Mb a nonequilibrium population of protein structures is generated that relaxes over a broad time range extending from picoseconds to milliseconds. This process is associated with migration of the ligand to cavities in the matrix and with a reduction in the geminate rebinding rate by several orders of magnitude. Here we report nanosecond time-resolved Laue diffraction data to 1.55-A resolution on a Mb mutant, which depicts the sequence of structural events associated with this extended relaxation. Motions of the distal E-helix, including the mutated residue Gln-64(E7), and of the CD-turn are found to lag significantly (100-300 ns) behind local rearrangements around the heme such as heme tilting, iron motion out of the heme plane, and swinging of the mutated residue Tyr-29(B10), all of which occur promptly (< or =3 ns). Over the same delayed time range, CO is observed to migrate from a cavity distal to the heme known to bind xenon (called Xe4) to another such cavity proximal to the heme (Xe1). We propose that the extended relaxation of the globin moiety reflects reequilibration among conformational substates known to play an essential role in controlling protein function.
Lingua originaleEnglish
pagine (da-a)8704-8709
Numero di pagine6
RivistaProceedings of the National Academy of Sciences
Stato di pubblicazionePubblicato - 2003

Keywords

  • CO photolysis
  • Laue diffraction
  • Myoglobin

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