Comparison of Different Matrices as Potential Quality Control Samples for Neurochemical Dementia Diagnostics

Natalia Lelental, Sebastian Brandner, Olga Kofanova, Kaj Blennow, Henrik Zetterberg, Ulf Andreasson, Sebastiaan Engelborghs, Barbara Mroczko, Tomasz Gabryelewicz, Charlotte Teunissen, Brit Mollenhauer, Lucilla Parnetti, Davide Chiasserini, Jose Luis Molinuevo, Armand Perret-Liaudet, Marcel M. Verbeek, Niels Andreasen, Frederic Brosseron, Justyna M.C. Bahl, Sanna-Kaisa HerukkaLucrezia Hausner, Lutz Frölich, Anne Labonte, Judes Poirier, Anne-Marie Miller, Norbert Zilka, Branislav Kovacech, Andrea Urbani, Silvia Suardi, Catarina Oliveira, Ines Baldeiras, Bruno Dubois, Uros Rot, Sylvain Lehmann, Anders Skinningsrud, Fay Betsou, Jens Wiltfang, Olymbia Gkatzima, Bengt Winblad, Michael Buchfelder, Johannes Kornhuber, Piotr Lewczuk

Risultato della ricerca: Contributo in rivistaArticolo in rivista

15 Citazioni (Scopus)


Aim. Lactulose/mannitol ratio is used to assess intestinal barrier function. Aim of this work was to develop a robust and rapid method for the analysis of lactulose and mannitol in urine by liquid chromatography coupled to tandem mass spectrometry. Lactulose/mannitol ratio has been measured in pediatric patients suffering from irritable bowel syndrome. Methods. Calibration curves and raffinose, used as internal standard, were prepared in water : acetonitrile 20:80. Fifty mu L of urine sample was added to 450 mu L of internal standard solution. The chromatographic separation was performed using a Luna NH2 column operating at a flow rate of 200 mu L/min and eluted with a linear gradient from 20% to 80% water in acetonitrile. Total run time is 9 minutes. The mass spectrometry operates in electrospray negative mode. Method was fully validated according to European Medicine Agency guidelines. Results and Conclusions. Linearity ranged from 10 to 1000 mg/L for mannitol and 2.5 to 1000 mg/L for lactulose. Imprecision in intra-and interassay was lower than 15% for both analytes. Accuracy was higher than 85%. Lactulose/mannitol ratio in pediatric patients is significantly higher than that measured in controls. The presented method, rapid and sensitive, is suitable in a clinical laboratory.
Lingua originaleEnglish
pagine (da-a)51-64
Numero di pagine14
RivistaJournal of Alzheimer's Disease
Stato di pubblicazionePubblicato - 2016


  • Alzheimer's disease
  • amyloid-beta


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