TY - JOUR
T1 - [Comparison of conventional culture methods and quantitative real-time PCR methods for the detection of Legionella pneumophila in water samples in a large University teaching hospital in Rome, Italy]
AU - Boccia, Stefania
AU - Laurenti, Patrizia
AU - Leoncini, Emanuele
AU - Amore, Rosarita
AU - Vincenti, Sara
AU - Arzani, Dario
AU - Berloco, Filippo
AU - Boninti, Federica
AU - Bruno, Stefania
AU - Celani, Fabrizio
AU - Damiani, Gianfranco
AU - Di Giannantonio, Paolo
AU - Moscato, Umberto
AU - Posteraro, Brunella
AU - Sezzatini, Romina
AU - Vecchioni, Alessia
AU - Wachocka, Malgorzata
AU - Ricciardi, Walter
AU - Quaranta, Gianluigi
AU - Ficarra, Maria Giovanna
PY - 2015
Y1 - 2015
N2 - The aims of this study were to identify the best threshold value for the real-time PCR method in detecting the presence of Legionella pneumophila in water samples, and to evaluate the prognostic significance of negative results obtained with the molecular method. From 2011 to 2014, 77 water samples were collected from hospital wards of a large University teaching hospital in Rome (Italy) and screened for L.pneumophila by the standard culture method and by real-time PCR. The high sensitivity and negative predictive value of real-time PCR make this method suitable as a quick screening tool to exclude the presence of L. pneumophila in water samples in the hospital setting.
AB - The aims of this study were to identify the best threshold value for the real-time PCR method in detecting the presence of Legionella pneumophila in water samples, and to evaluate the prognostic significance of negative results obtained with the molecular method. From 2011 to 2014, 77 water samples were collected from hospital wards of a large University teaching hospital in Rome (Italy) and screened for L.pneumophila by the standard culture method and by real-time PCR. The high sensitivity and negative predictive value of real-time PCR make this method suitable as a quick screening tool to exclude the presence of L. pneumophila in water samples in the hospital setting.
KW - Legionella pneumophila
KW - quantitative real-time PCR
KW - Legionella pneumophila
KW - quantitative real-time PCR
UR - http://hdl.handle.net/10807/72397
M3 - Articolo in rivista
SN - 0019-1639
VL - 71
SP - 569
EP - 576
JO - IGIENE E SANITÀ PUBBLICA
JF - IGIENE E SANITÀ PUBBLICA
ER -