TY - JOUR
T1 - Comparing different methods for homocysteine determination
AU - Zappacosta, Bruno
AU - Persichilli, Silvia
AU - Scribano, Donata
AU - Minucci, Angelo
AU - Lazzaro, Daniele
AU - De Sole, Pasquale
AU - Giardina, Bruno
PY - 2002
Y1 - 2002
N2 - Slightly elevated values of homocysteine are commonly associated with thromboembolic diseases, while high values can be found in patients with congenital metabolic defects or nutritional problems. The clinical use of homocysteine as an independent marker of cardiovascular disease was limited in the past by technical problems with its measurement, the instrumentation (HPLC, radioenzymatic assays, gas chromatography-mass spectrometry, etc.) and the necessary skills required. Commercially available immunoassays now permit a simpler and more rapid measurement of homocysteine, that is more suitable for routine clinical laboratories; in this paper we analyze the results obtained by using three fully automated methods for homocysteine determination (Abbott IMx immunoassay, Abbott AxSYM immunoassay and Immulite 2000 homocysteine immunoassay) and their correlation with the widely used HPLC method. The results clearly indicate that all three automated immunochemical methods correlate well with the HPLC method (slope 0.97-1.03; intercept 0.95-1.91 with a recovery above 95% for all three methods).
AB - Slightly elevated values of homocysteine are commonly associated with thromboembolic diseases, while high values can be found in patients with congenital metabolic defects or nutritional problems. The clinical use of homocysteine as an independent marker of cardiovascular disease was limited in the past by technical problems with its measurement, the instrumentation (HPLC, radioenzymatic assays, gas chromatography-mass spectrometry, etc.) and the necessary skills required. Commercially available immunoassays now permit a simpler and more rapid measurement of homocysteine, that is more suitable for routine clinical laboratories; in this paper we analyze the results obtained by using three fully automated methods for homocysteine determination (Abbott IMx immunoassay, Abbott AxSYM immunoassay and Immulite 2000 homocysteine immunoassay) and their correlation with the widely used HPLC method. The results clearly indicate that all three automated immunochemical methods correlate well with the HPLC method (slope 0.97-1.03; intercept 0.95-1.91 with a recovery above 95% for all three methods).
KW - homocysteine, immunoassay, HPLC
KW - homocysteine, immunoassay, HPLC
UR - http://hdl.handle.net/10807/15800
UR - http://www.scopus.com/inward/record.url?eid=2-s2.0-0036914938&partnerid=40&md5=8721349722c99f9c66fbd2ebafe45a9e
U2 - 10.1515/CCLM.2002.200
DO - 10.1515/CCLM.2002.200
M3 - Article
SN - 1434-6621
VL - 40
SP - 1139
EP - 1142
JO - Clinical Chemistry and Laboratory Medicine
JF - Clinical Chemistry and Laboratory Medicine
ER -