TY - JOUR
T1 - Comparative “phenol-omics” and gene expression analyses in peach (Prunus persica) skin in response to different postharvest UV-B treatments
AU - Santin, Marco
AU - Lucini, Luigi
AU - Castagna, Antonella
AU - Rocchetti, Gabriele
AU - Hauser, Marie-Theres
AU - Ranieri, Annamaria
PY - 2019
Y1 - 2019
N2 - Ultraviolet-B (UV–B) radiation impacts the plant behaviour in many ways, including modifying their secondary metabolism. Although several studies have quantified the UV-B effects on phenolic composition, most of them focused on leaves or investigated a limited amount of phenolics. The present work aimed to investigate the phenolic changes after two postharvest UV-B treatments, 10 and 60 min (1.39 kJ m−2 and 8.33 kJ m−2, respectively), on peach (Prunus persica cv Fairtime) fruit with a non-targeted, whole profiling approach, and targeted gene expression analysis on skin. After both UV-B exposures, peach fruit were harvested at 24 and 36 h for “phenol-omics” analysis, while additional 6 h and 12 h recovery times were used for gene expression analysis. Our results revealed that both UV-B exposures resulted in a decrease of several phenolic compounds, such as anthocyanins, after 24 h from the exposure. In contrast, the expression of the UV-B signalling components, the phenylpropanoid biosynthesis genes and their transcriptional regulators increased 6 h after the treatment, mostly with a UV-B-dose dependent behaviour, preceding an accumulation of most phenolics in both the UV-B treatments at 36 h compared to 24 h. Orthogonal projections to latent structures discriminant analysis (OPLS-DA) revealed that flavonoids, particularly anthocyanins, were the main phenolic subclasses accumulated after UV-B exposure.
AB - Ultraviolet-B (UV–B) radiation impacts the plant behaviour in many ways, including modifying their secondary metabolism. Although several studies have quantified the UV-B effects on phenolic composition, most of them focused on leaves or investigated a limited amount of phenolics. The present work aimed to investigate the phenolic changes after two postharvest UV-B treatments, 10 and 60 min (1.39 kJ m−2 and 8.33 kJ m−2, respectively), on peach (Prunus persica cv Fairtime) fruit with a non-targeted, whole profiling approach, and targeted gene expression analysis on skin. After both UV-B exposures, peach fruit were harvested at 24 and 36 h for “phenol-omics” analysis, while additional 6 h and 12 h recovery times were used for gene expression analysis. Our results revealed that both UV-B exposures resulted in a decrease of several phenolic compounds, such as anthocyanins, after 24 h from the exposure. In contrast, the expression of the UV-B signalling components, the phenylpropanoid biosynthesis genes and their transcriptional regulators increased 6 h after the treatment, mostly with a UV-B-dose dependent behaviour, preceding an accumulation of most phenolics in both the UV-B treatments at 36 h compared to 24 h. Orthogonal projections to latent structures discriminant analysis (OPLS-DA) revealed that flavonoids, particularly anthocyanins, were the main phenolic subclasses accumulated after UV-B exposure.
KW - Anthocyanins
KW - Flavonoids
KW - Fruit
KW - Gene Expression Regulation, Plant
KW - Genetics
KW - Phenylpropanoid biosynthesis
KW - Physiology
KW - Plant Epidermis
KW - Plant Science
KW - Prunus persica
KW - Real-Time Polymerase Chain Reaction
KW - Secondary metabolites
KW - Transcriptome
KW - UV radiation
KW - UVR8
KW - Ultraviolet Rays
KW - Anthocyanins
KW - Flavonoids
KW - Fruit
KW - Gene Expression Regulation, Plant
KW - Genetics
KW - Phenylpropanoid biosynthesis
KW - Physiology
KW - Plant Epidermis
KW - Plant Science
KW - Prunus persica
KW - Real-Time Polymerase Chain Reaction
KW - Secondary metabolites
KW - Transcriptome
KW - UV radiation
KW - UVR8
KW - Ultraviolet Rays
UR - http://hdl.handle.net/10807/133309
UR - http://www.journals.elsevier.com/plant-physiology-and-biochemistry/
U2 - 10.1016/j.plaphy.2018.11.009
DO - 10.1016/j.plaphy.2018.11.009
M3 - Article
SN - 0981-9428
VL - 135
SP - 511
EP - 519
JO - Plant Physiology and Biochemistry
JF - Plant Physiology and Biochemistry
ER -