Comparative “phenol-omics” and gene expression analyses in peach (Prunus persica) skin in response to different postharvest UV-B treatments

Luigi Lucini, Gabriele Rocchetti, Marco Santin, Antonella Castagna, Marie-Theres Hauser, Annamaria Ranieri

Risultato della ricerca: Contributo in rivistaArticolo in rivista

8 Citazioni (Scopus)

Abstract

Ultraviolet-B (UV–B) radiation impacts the plant behaviour in many ways, including modifying their secondary metabolism. Although several studies have quantified the UV-B effects on phenolic composition, most of them focused on leaves or investigated a limited amount of phenolics. The present work aimed to investigate the phenolic changes after two postharvest UV-B treatments, 10 and 60 min (1.39 kJ m−2 and 8.33 kJ m−2, respectively), on peach (Prunus persica cv Fairtime) fruit with a non-targeted, whole profiling approach, and targeted gene expression analysis on skin. After both UV-B exposures, peach fruit were harvested at 24 and 36 h for “phenol-omics” analysis, while additional 6 h and 12 h recovery times were used for gene expression analysis. Our results revealed that both UV-B exposures resulted in a decrease of several phenolic compounds, such as anthocyanins, after 24 h from the exposure. In contrast, the expression of the UV-B signalling components, the phenylpropanoid biosynthesis genes and their transcriptional regulators increased 6 h after the treatment, mostly with a UV-B-dose dependent behaviour, preceding an accumulation of most phenolics in both the UV-B treatments at 36 h compared to 24 h. Orthogonal projections to latent structures discriminant analysis (OPLS-DA) revealed that flavonoids, particularly anthocyanins, were the main phenolic subclasses accumulated after UV-B exposure.
Lingua originaleEnglish
pagine (da-a)511-519
Numero di pagine9
RivistaPlant Physiology and Biochemistry
Volume135
DOI
Stato di pubblicazionePubblicato - 2019

Keywords

  • Anthocyanins
  • Flavonoids
  • Fruit
  • Gene Expression Regulation, Plant
  • Genetics
  • Phenylpropanoid biosynthesis
  • Physiology
  • Plant Epidermis
  • Plant Science
  • Prunus persica
  • Real-Time Polymerase Chain Reaction
  • Secondary metabolites
  • Transcriptome
  • UV radiation
  • UVR8
  • Ultraviolet Rays

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