Comparative Evaluation of the Bruker Biotyper and Vitek MS Matrix-Assisted Laser Desorption Ionization-Time Of Flight (MALDI-TOF) Mass Spectrometry Systems for Identification of Yeasts of Medical Importance

Brunella Posteraro; Maurizio Sanguinetti; Elena De Carolis; Antonietta Vella; Luisa Vaccaro; Alberto Ruggeri; Nicasio Mancini; Laura Infurnari; Nicola Clementi; Roberto Burioni; Massimo Clementi

doi:10.1128/JCM.00841-13

Comparative Evaluation of the Bruker Biotyper and Vitek MS Matrix-Assisted Laser Desorption Ionization-Time Of Flight (MALDI-TOF) Mass Spectrometry Systems for Identification of Yeasts of Medical Importance

Brunella Posteraro, Maurizio Sanguinetti, Elena De Carolis, Antonietta Vella, Luisa Vaccaro, Alberto Ruggeri, Nicasio Mancini, Laura Infurnari, Nicola Clementi, Roberto Burioni, Massimo Clementi

Risultato della ricerca: Contributo in rivista › Articolo in rivista › peer review

71 Citazioni (Scopus)

Abstract

We report the first comparative evaluation between the Bruker Biotyper MS (BMS) and the Vitek MS (VMS) for the identification of yeasts. The rate of correct identifications at the species level was comparable using the commercial databases (89.8% versus 84.3%; P = 0.712), but higher for BMS using an in-house-extended database (100% versus 84.3%; P = 0.245). Importantly, the rate of misidentification was significantly higher for VMS (1% versus 12.1%; P < 0.0001), including the rate of major errors (0% versus 4.5%; P = 0.0036).

Lingua originale	English
pagine (da-a)	2453-2457
Numero di pagine	5
Rivista	Journal of Clinical Microbiology
Volume	51
DOI	https://doi.org/10.1128/JCM.00841-13
Stato di pubblicazione	Pubblicato - 2013

Keywords

MALDI

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10.1128/JCM.00841-13

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Posteraro, B., Sanguinetti, M., De Carolis, E., Vella, A., Vaccaro, L., Ruggeri, A., Mancini, N., Infurnari, L., Clementi, N., Burioni, R., & Clementi, M. (2013). Comparative Evaluation of the Bruker Biotyper and Vitek MS Matrix-Assisted Laser Desorption Ionization-Time Of Flight (MALDI-TOF) Mass Spectrometry Systems for Identification of Yeasts of Medical Importance. Journal of Clinical Microbiology, 51, 2453-2457. https://doi.org/10.1128/JCM.00841-13

Posteraro, Brunella ; Sanguinetti, Maurizio ; De Carolis, Elena ; Vella, Antonietta ; Vaccaro, Luisa ; Ruggeri, Alberto ; Mancini, Nicasio ; Infurnari, Laura ; Clementi, Nicola ; Burioni, Roberto ; Clementi, Massimo. / Comparative Evaluation of the Bruker Biotyper and Vitek MS Matrix-Assisted Laser Desorption Ionization-Time Of Flight (MALDI-TOF) Mass Spectrometry Systems for Identification of Yeasts of Medical Importance. In: Journal of Clinical Microbiology. 2013 ; Vol. 51. pagg. 2453-2457.

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title = "Comparative Evaluation of the Bruker Biotyper and Vitek MS Matrix-Assisted Laser Desorption Ionization-Time Of Flight (MALDI-TOF) Mass Spectrometry Systems for Identification of Yeasts of Medical Importance",

abstract = "We report the first comparative evaluation between the Bruker Biotyper MS (BMS) and the Vitek MS (VMS) for the identification of yeasts. The rate of correct identifications at the species level was comparable using the commercial databases (89.8% versus 84.3%; P = 0.712), but higher for BMS using an in-house-extended database (100% versus 84.3%; P = 0.245). Importantly, the rate of misidentification was significantly higher for VMS (1% versus 12.1%; P < 0.0001), including the rate of major errors (0% versus 4.5%; P = 0.0036).",

keywords = "MALDI, MALDI",

author = "Brunella Posteraro and Maurizio Sanguinetti and {De Carolis}, Elena and Antonietta Vella and Luisa Vaccaro and Alberto Ruggeri and Nicasio Mancini and Laura Infurnari and Nicola Clementi and Roberto Burioni and Massimo Clementi",

year = "2013",

doi = "10.1128/JCM.00841-13",

language = "English",

volume = "51",

pages = "2453--2457",

journal = "Journal of Clinical Microbiology",

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publisher = "American Society for Microbiology / DC:1752 N Street Northwest:Washington, DC 20036:(202)737-3600, EMAIL: jadelman@asmusa.org, INTERNET: http://www.asmusa.org/asm.htm, Fax: (202)942-9342",

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Posteraro, B , Sanguinetti, M, De Carolis, E, Vella, A, Vaccaro, L, Ruggeri, A, Mancini, N, Infurnari, L, Clementi, N, Burioni, R & Clementi, M 2013, 'Comparative Evaluation of the Bruker Biotyper and Vitek MS Matrix-Assisted Laser Desorption Ionization-Time Of Flight (MALDI-TOF) Mass Spectrometry Systems for Identification of Yeasts of Medical Importance', Journal of Clinical Microbiology, vol. 51, pagg. 2453-2457. https://doi.org/10.1128/JCM.00841-13

Comparative Evaluation of the Bruker Biotyper and Vitek MS Matrix-Assisted Laser Desorption Ionization-Time Of Flight (MALDI-TOF) Mass Spectrometry Systems for Identification of Yeasts of Medical Importance. / Posteraro, Brunella ; Sanguinetti, Maurizio; De Carolis, Elena; Vella, Antonietta; Vaccaro, Luisa; Ruggeri, Alberto; Mancini, Nicasio; Infurnari, Laura; Clementi, Nicola; Burioni, Roberto; Clementi, Massimo.

In: Journal of Clinical Microbiology, Vol. 51, 2013, pag. 2453-2457.

Risultato della ricerca: Contributo in rivista › Articolo in rivista › peer review

TY - JOUR

T1 - Comparative Evaluation of the Bruker Biotyper and Vitek MS Matrix-Assisted Laser Desorption Ionization-Time Of Flight (MALDI-TOF) Mass Spectrometry Systems for Identification of Yeasts of Medical Importance

AU - Posteraro, Brunella

AU - Sanguinetti, Maurizio

AU - De Carolis, Elena

AU - Vella, Antonietta

AU - Vaccaro, Luisa

AU - Ruggeri, Alberto

AU - Mancini, Nicasio

AU - Infurnari, Laura

AU - Clementi, Nicola

AU - Burioni, Roberto

AU - Clementi, Massimo

PY - 2013

Y1 - 2013

N2 - We report the first comparative evaluation between the Bruker Biotyper MS (BMS) and the Vitek MS (VMS) for the identification of yeasts. The rate of correct identifications at the species level was comparable using the commercial databases (89.8% versus 84.3%; P = 0.712), but higher for BMS using an in-house-extended database (100% versus 84.3%; P = 0.245). Importantly, the rate of misidentification was significantly higher for VMS (1% versus 12.1%; P < 0.0001), including the rate of major errors (0% versus 4.5%; P = 0.0036).

AB - We report the first comparative evaluation between the Bruker Biotyper MS (BMS) and the Vitek MS (VMS) for the identification of yeasts. The rate of correct identifications at the species level was comparable using the commercial databases (89.8% versus 84.3%; P = 0.712), but higher for BMS using an in-house-extended database (100% versus 84.3%; P = 0.245). Importantly, the rate of misidentification was significantly higher for VMS (1% versus 12.1%; P < 0.0001), including the rate of major errors (0% versus 4.5%; P = 0.0036).

KW - MALDI

UR - http://hdl.handle.net/10807/48093

U2 - 10.1128/JCM.00841-13

DO - 10.1128/JCM.00841-13

M3 - Article

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SP - 2453

EP - 2457

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

ER -

Posteraro B , Sanguinetti M, De Carolis E, Vella A, Vaccaro L, Ruggeri A e altri. Comparative Evaluation of the Bruker Biotyper and Vitek MS Matrix-Assisted Laser Desorption Ionization-Time Of Flight (MALDI-TOF) Mass Spectrometry Systems for Identification of Yeasts of Medical Importance. Journal of Clinical Microbiology. 2013;51:2453-2457. https://doi.org/10.1128/JCM.00841-13

Comparative Evaluation of the Bruker Biotyper and Vitek MS Matrix-Assisted Laser Desorption Ionization-Time Of Flight (MALDI-TOF) Mass Spectrometry Systems for Identification of Yeasts of Medical Importance

Abstract

Keywords

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