Collaborative national multicenter for the identification of conversion factors from copies/mL to international units/mL for the normalization of HCMV DNA load

Rosaria Santangelo, Maurizio Sanguinetti, Francesca Sidoti, Antonio Piralla, Cristina Costa, Maria Luisa Scarasciulli, Agata Calvario, Pier Giulio Conaldi, Pierpaolo Paba, Carlo Federico Perno, Aurelia Gaeta, Guido Antonelli, Giuseppe Sodano, Maria Linda Vatteroni, Luisa Barzon, Giorgio Palù, Isabella Abbate, Maria Rosaria Capobianchi, Giulia Piccirilli, Tiziana LazzarottoFausto Baldanti, Rossana Cavallo

Risultato della ricerca: Contributo in rivistaArticolo in rivista

5 Citazioni (Scopus)

Abstract

The present multicentric (n = 11 laboratories) study aimed to identify conversion factors from copies/mL to international units (IU)/mL for the normalization of HCMV DNA load using the first WHO International Standard for HCMV nucleic acid amplification techniques and to enhance interlaboratory agreement of HCMV DNA quantification methods. Study protocols for whole blood and plasma (extraction and amplification) were performed to calculate conversion factors from HCMV DNA copy number to IU. The greatest variability was observed in samples with lower HCMV concentrations (3.0 Log10) in both biological matrices. Overall, 73.1% (206/282) of whole blood and 82.2% (324/394) of plasma samples analyzed fell within an acceptable variation range (±0.5 Log10 difference). An average of 0.64 (range 0.21–1.17) was the conversion factor calculated for the HCMV whole blood panel and 0.82 (range 0.39–2.2) for the HCMV plasma panel.
Lingua originaleEnglish
pagine (da-a)152-158
RivistaDiagnostic Microbiology and Infectious Disease
Volume95
DOI
Stato di pubblicazionePubblicato - 2019

Keywords

  • HCMV
  • International standard
  • Multicenter study

Fingerprint

Entra nei temi di ricerca di 'Collaborative national multicenter for the identification of conversion factors from copies/mL to international units/mL for the normalization of HCMV DNA load'. Insieme formano una fingerprint unica.

Cita questo