Clinical significance of NOD2/CARD15 and Toll-like receptor 4 gene single nucleotide polymorphisms in inflammatory bowel disease

Giovanna Elisa Calabro', Luciana Rigoli, Claudio Romano, Rosario Alberto Caruso, Maria A Lo Presti, Chiara Di Bella, Vincenzo Procopio, Giuseppina Lo Giudice, Maria Amorini, Giuseppe Costantino, Maria D. Sergi, Caterina Cuppari, Romina Gallizzi, Carmelo Damiano Salpietro, Walter Fries

Risultato della ricerca: Contributo in rivistaArticolo in rivista

Abstract

Aim: To evaluate the role of genetic factors in the pathogenesis of Crohn's disease (CD) and ulcerative colitis (UC), we investigated the single nucleotide polymorphisms (SNPs) of NOD2/CARD15 (R702W, G908R and L1007finsC), and Toll-like receptor 4 (TLR4) genes (D299G and T399I) in a selected inflammatory bowel disease (IBD) population coming from Southern Italy. Methods: Allele and genotype frequencies of NOD2/CARD15 (R702W, G908R and L1007finsC) and TLR4 (D299G and T399I) SNPs were examined in 133 CD patients, in 45 UC patients, and in 103 healthy controls. A genotype-phenotype correlation was performed. Results: NOD2/CARD15 R702W mutation was significantly more frequent in CD (9.8%) than in controls (2.4%, P = 0.001) and in UC (2.3%, P = 0.03). No significant difference was found between UC patients and control group (P > 0.05). In CD and UC patients, no significant association with G908R variant was found. L1007finsC SNP showed an association with CD (9.8%) compared with controls (2.9%, P = 0.002) and UC patients (2.3%, P = 0.01). Moreover, in CD patients, G908R and L1007finsC mutations were significantly associated with different phenotypes compared to CD wild-type patients. No association of IBD with the TLR4 SNPs was found in either cohort (allele frequencies: D299G-controls 3.9%, CD 3.7%, UC 3.4%, P > 0.05; T399I-controls 2.9%, CD 3.0%, UC 3.4%, P > 0.05). Conclusion: These findings confirm that, in our IBD patients selected from Southern Italy, the NOD2/CARD15, but not TLR4 SNPs, are associated with increased risk of CD. © 2008 The WJG Press. All rights reserved.
Lingua originaleEnglish
pagine (da-a)4454-4461
Numero di pagine8
RivistaWorld Journal of Gastroenterology
Volume2008
DOI
Stato di pubblicazionePubblicato - 2008

Keywords

  • Adult
  • Case-Control Studies
  • Colitis, Ulcerative
  • Crohn Disease
  • Crohn's disease
  • Female
  • Gene Frequency
  • Genetic Predisposition to Disease
  • Genotype
  • Humans
  • Inflammatory Bowel Diseases
  • Italy
  • Male
  • Middle Aged
  • NOD2/CARD15 gene
  • Nod2 Signaling Adaptor Protein
  • Phenotype
  • Polymorphism, Single Nucleotide
  • Risk Factors
  • Single nucleotide polymorphisms
  • Toll-Like Receptor 4
  • Toll-like receptor 4 gene
  • Ulcerative colitis

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