Clinical and biological role of secretory phospholipase A2 in acute respiratory distress syndrome infants

Giorgio Conti, Marco Piastra, Massimo Antonelli, Daniele De Luca, Angelo Minucci, Francesca Vendittelli, Leonarda Gentile, Eleonora Stival, Ettore Domenico Capoluongo, Elena Lopez-Rodriguez, Mercedes Echaide, Jesus Perez-Gil

Risultato della ricerca: Contributo in rivistaArticolo in rivista

30 Citazioni (Scopus)

Abstract

Introduction: Secretory phospholipase A2 is supposed to play a role in acute lung injury but no data are available for pediatric acute respiratory distress syndrome (ARDS). It is not clear which enzyme subtypes are secreted and what the relationships are between enzyme activity, biophysical and biochemical parameters, and clinical outcomes. We aimed to measure the enzyme and identify its subtypes and to study its biochemical and biophysical effect. The secondary aim was to correlate enzyme activity with clinical outcome. Methods: Bronchoalveolar lavage was performed in 24 infants with ARDS and 14 controls with no lung disease. Samples were assayed for secretory phospholipase A2 and molecules related to its activity and expression. Western blotting and captive bubble surfactometry were also performed. Clinical data were real time downloaded. Results: Tumor necrosis factor-alpha (814 (506-2,499) vs. 287 (111-1,315) pg/mL; P = 0.04), enzyme activity (430 (253600) vs. 149 (61-387) IU/mL; P = 0.01), free fatty acids (4.3 (2.8-8.6) vs. 2 (0.8-4.6) mM; P = 0.026), and minimum surface tension (25.6 +/- 6.1 vs. 18 +/- 1.8 mN/m; P = 0.006) were higher in ARDS than in controls. Phospholipids are lower in ARDS than in controls (76.5 (54-100) vs. 1,094 (536-2,907) mu g/mL; P = 0.0001). Three enzyme subtypes were identified (-IIA, -V, -X), although in lower quantities in controls; another subtype (-IB) was mainly detected in ARDS. Significant correlations exist between enzyme activity, free fatty acids (rho = 0.823; P < 0.001), and surface tension (rho = 0.55; P < 0.028). Correlations also exist with intensive care stay (rho = 0.54; P = 0.001), PRISM-III24 (rho = 0.79; P < 0.001), duration of ventilation (rho = 0.53; P = 0.002), and oxygen therapy (rho = 0.54; P = 0.001). Conclusions: Secretory phospholipase A2 activity is raised in pediatric ARDS and constituted of four subtypes. Enzyme correlates with some inflammatory mediators, surface tension, and major clinical outcomes. Secretory phospholipase A2 may be a clinically relevant target in pediatric ARDS.
Lingua originaleEnglish
pagine (da-a)N/A-N/A
Numero di pagine12
RivistaCritical Care
Volume17
DOI
Stato di pubblicazionePubblicato - 2013

Keywords

  • BRONCHOALVEOLAR LAVAGE
  • LUNG INJURY

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