TY - JOUR
T1 - CISH the FISH for HER2: our laboratory experience.
AU - Arena, Vincenzo
AU - Pennacchia, Ilaria
AU - Vecchio, Fabio Maria
AU - Carbone, Arnaldo
PY - 2010
Y1 - 2010
N2 - We read with great interest the article by Hoff et al. on the applicability of visualization of fluorescence in situ hybridization (FISH) probes by dual-color chromogenic in situ hybridization (CISH) and totally agree about the need to perform studies on a larger number of samples to confirm their data. In our laboratory, we have been using this technique for 1 year, and to date our sample is composed of 40 cases tested for HER2/centromere 17 status by FISH and subsequent CISH conversion. In our sample, the concordance between FISH and CISH assays is 100%, in complete agreement with the results reported by Hoff et al. As a matter of fact, as we show in the figure, all pairs of ratio values were consistently above or under the cutoff in every case.
AB - We read with great interest the article by Hoff et al. on the applicability of visualization of fluorescence in situ hybridization (FISH) probes by dual-color chromogenic in situ hybridization (CISH) and totally agree about the need to perform studies on a larger number of samples to confirm their data. In our laboratory, we have been using this technique for 1 year, and to date our sample is composed of 40 cases tested for HER2/centromere 17 status by FISH and subsequent CISH conversion. In our sample, the concordance between FISH and CISH assays is 100%, in complete agreement with the results reported by Hoff et al. As a matter of fact, as we show in the figure, all pairs of ratio values were consistently above or under the cutoff in every case.
KW - breast
KW - cancer
KW - breast
KW - cancer
UR - http://hdl.handle.net/10807/63511
UR - http://ajcp.ascpjournals.org/content/134/2/347.long
M3 - Article
SN - 0002-9173
VL - 134
SP - 347
EP - 348
JO - American Journal of Clinical Pathology
JF - American Journal of Clinical Pathology
ER -