Chromographic Analysis and Cytotoxic Effects of Chlorhexidine and Sodium Hypochlorite Reaction Mixtures

Giuseppina Nocca, Hany Mohamed Aly Ahmed, Giuseppe Ettore Martorana, Cinzia Anna Maria Calla', Gianluca Gambarini, Sandro Rengo, Gianrico Spagnuolo

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

11 Citazioni (Scopus)


Introduction: The literature reveals controversies regarding the formation of para-chloroaniline (PCA) when chlorhexidine (CHX) is mixed with sodium hypochlorite (NaOCl). This study aimed to investigate the stability of PCA in the presence of NaOCl and to examine the in vitro cytotoxic effects of CHX/NaOCl reaction mixtures. Methods: Different volumes of NaOCl were added to CHX (mix 1) or PCA (mix 2). Upon centrifugation, the supernatant and precipitate fractions collected from samples were analyzed using high-performance liquid chromatography. The cytotoxic effects of both fractions were examined on human periodontal ligament and 3T3 fibroblast cell lines. Results: High-performance liquid chromatographic analysis showed no PCA signal when NaOCl was mixed with CHX (mix 1). In mix 2, the intensity of PCA was decreased when NaOCl was added to PCA, and chromatographic signals, similar to that of CHX/NaOCl, were also observed. The mortality of precipitates exerted on both cell lines was lower compared with that of supernatants. Conclusions: The discrepancy in the data from the literature could be caused by the instability of the PCA in the presence of NaOCl. The CHX/NaOCl reaction mixture exhibits a wide range of cytotoxic effects.
Lingua originaleEnglish
pagine (da-a)30531-30539
Numero di pagine9
RivistaJournal of Endodontics
Stato di pubblicazionePubblicato - 2017


  • Brown precipitate
  • Chlorhexidine
  • Cytotoxicity
  • Dentistry (all)
  • Para-chloroaniline
  • Sodium hypochlorite


Entra nei temi di ricerca di 'Chromographic Analysis and Cytotoxic Effects of Chlorhexidine and Sodium Hypochlorite Reaction Mixtures'. Insieme formano una fingerprint unica.

Cita questo