TY - JOUR
T1 - Characterization of the human salivary basic proline-rich protein complex by a proteomic approach
AU - Messana, Irene
AU - Cabras, Tiziana
AU - Inzitari, Rosanna
AU - Lupi, Alessandro
AU - Zuppi, Cecilia
AU - Olmi, Chiara
AU - Fadda, Maria Benedetta
AU - Cordaro, Massimo
AU - Giardina, Bruno
AU - Castagnola, Massimo
PY - 2004
Y1 - 2004
N2 - Thirteen samples of human normal whole saliva were analyzed by RP-HPLC-ESI-MS and MALDI-TOF-MS to investigate the basic proline-rich protein complex. Between known basic-PRPs the P-B, P-C (or IB-8b), P-D (or IB-5), P-E (or IB-9), P-F (or IB-8), P-H (or IB-4), IB-6,II-2, IB-1, and IB-8a glucosylated were identified, whereas the II-1, IB-7, PA, and D1-A peptides were not detected. Some detected masses not attributable to known basic-PRPs were putatively ascribed to II-2 and IB-1 nonphosphorylated, II-2 and IB-1 missing the C-terminal arginine residue, and the 1-62 fragment of IB-6, named P-J peptide. A correlation matrix analysis revealed a cluster of correlation among all the basic PRF's (apart from the P-B peptide) which is in agreement with their common parotid origin.
AB - Thirteen samples of human normal whole saliva were analyzed by RP-HPLC-ESI-MS and MALDI-TOF-MS to investigate the basic proline-rich protein complex. Between known basic-PRPs the P-B, P-C (or IB-8b), P-D (or IB-5), P-E (or IB-9), P-F (or IB-8), P-H (or IB-4), IB-6,II-2, IB-1, and IB-8a glucosylated were identified, whereas the II-1, IB-7, PA, and D1-A peptides were not detected. Some detected masses not attributable to known basic-PRPs were putatively ascribed to II-2 and IB-1 nonphosphorylated, II-2 and IB-1 missing the C-terminal arginine residue, and the 1-62 fragment of IB-6, named P-J peptide. A correlation matrix analysis revealed a cluster of correlation among all the basic PRF's (apart from the P-B peptide) which is in agreement with their common parotid origin.
KW - PEPTIDE
KW - SALIVARY
KW - PEPTIDE
KW - SALIVARY
UR - http://hdl.handle.net/10807/25605
M3 - Article
SN - 1535-3893
SP - 792
EP - 800
JO - Journal of Proteome Research
JF - Journal of Proteome Research
ER -