Abstract

Ascorbic acid, isoascorbic acid and dehydroascorbic acid inhibit bovine kidney alkaline phosphatase activity. Ascorbic acid free radicals seem not to be involved. Dialysis does not make the inactivation reversible. A competitive mechanism can be inferred from experiments with phosphate and substrates, which block the activity decay. The influence of temperature, pH, other inhibitors and tertiary structure modifications on the inactivation process is also investigated.
Lingua originaleEnglish
pagine (da-a)343-346
Numero di pagine4
RivistaBIOCHIMICA ET BIOPHYSICA ACTA
Volume789
Stato di pubblicazionePubblicato - 1984

Keywords

  • Alkaline Phosphatase
  • Animals
  • Ascorbic Acid
  • Binding, Competitive
  • Cattle
  • Dehydroascorbic Acid
  • Hydrogen-Ion Concentration
  • Kidney
  • Structure-Activity Relationship
  • Temperature

Fingerprint

Entra nei temi di ricerca di 'Characterization of alkaline phosphatase inactivation by ascorbic acid'. Insieme formano una fingerprint unica.

Cita questo