CD8α+ dendritic cells prime TCR-peptide-reactive regulatory CD4+FOXP3- T cells

Risultato della ricerca: Contributo in rivistaArticolo in rivista

8 Citazioni (Scopus)

Abstract

CD4+ T cells with immune regulatory function can be either FOXP3+ or FOXP3-. We have previously shown that priming of naturally occurring TCR-peptide-reactive CD4+FOXP3- Treg specifically controls Vβ8.2+CD4+ T cells mediating EAE. However, the mechanism by which these Treg are primed to recognize their cognate antigenic determinant, which is derived from the TCRVβ8.2-chain, is not known. In this study we show that APC derived from splenocytes of naïve mice are able to stimulate cloned CD4+ Treg in the absence of exogenous antigen, and their stimulation capacity is augmented during EAE. Among the APC populations, DC were the most efficient in stimulating the Treg. Stimulation of CD4+ Treg was dependent upon processing and presentation of TCR peptides from ingested Vβ8.2TCR+CD4 + T cells. Additionally, DC pulsed with TCR peptide or apoptotic Vβ8.2+ T cells were able to prime Treg in vivo and mediate protection from disease in a CD8-dependent fashion. These data highlight a novel mechanism for the priming of CD4+ Treg by CD8α+ DC and suggest a pathway that can be exploited to prime antigen-specific regulation of T-cell-mediated inflammatory disease. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.
Lingua originaleEnglish
pagine (da-a)1906-1915
Numero di pagine10
RivistaEuropean Journal of Immunology
Volume40
DOI
Stato di pubblicazionePubblicato - 2010

Keywords

  • Animals
  • Antigen Presentation
  • Antigen presentation/processing
  • Apoptosis
  • CD4 Antigens
  • CD8 Antigens
  • Clone Cells
  • DC
  • Dendritic Cells
  • EAE/MS
  • Encephalomyelitis, Autoimmune, Experimental
  • Female
  • Forkhead Transcription Factors
  • Humans
  • Immunization
  • Immunology
  • Immunology and Allergy
  • Lymphocyte Activation
  • Mice
  • Mice, Inbred Strains
  • Myelin Basic Protein
  • Peptide Fragments
  • Receptors, Antigen, T-Cell, alpha-beta
  • T-Cell Antigen Receptor Specificity
  • T-Lymphocytes, Regulatory
  • TCR
  • Tolerance/suppression/anergy

Fingerprint

Entra nei temi di ricerca di 'CD8α+ dendritic cells prime TCR-peptide-reactive regulatory CD4+FOXP3- T cells'. Insieme formano una fingerprint unica.

Cita questo