TY - JOUR
T1 - CD147 Promotes Cell Small Extracellular Vesicles Release during Colon Cancer Stem Cells Differentiation and Triggers Cellular Changes in Recipient Cells
AU - Lucchetti, Donatella
AU - Colella, Filomena
AU - Perelli, Luigi
AU - Ricciardi Tenore, Claudio
AU - Calapa', Federica
AU - Fiori, Micol E.
AU - Fiori, Micol Eleonora
AU - Carbone, Federica
AU - De Maria Marchiano, Ruggero
AU - Sgambato, Alessandro
PY - 2020
Y1 - 2020
N2 - Cancer cells secrete small extracellular vesicles (sEVs) that are involved in the remodeling of tumor microenvironment (TME) and can promote tumor progression. The role of sEVs and their molecular key players in colon cancer stem cells differentiation are poorly understood. This study aimed to analyze the role and content of sEVs released during the differentiation of colorectal cancer stem cells. Here we show that sEVs secretion during colon cancer stem cells differentiation is partially controlled by CD147, a well-known player involved in colon cancer tumorigenesis. CD147 + sEVs activate a signaling cascade in recipient cells inducing molecular invasive features in colon cancer cells. CD147 knockdown as well as anti-CD147 antibodies impaired sEVs release and downstream effects on recipient cells and blocking multivesicular body maturation prevented sEVs release during the differentiation. Our findings reveal a functional role of CD147 in promoting sEVs release during the differentiation of colon cancer stem cells and in triggering cellular changes in recipient cells.
AB - Cancer cells secrete small extracellular vesicles (sEVs) that are involved in the remodeling of tumor microenvironment (TME) and can promote tumor progression. The role of sEVs and their molecular key players in colon cancer stem cells differentiation are poorly understood. This study aimed to analyze the role and content of sEVs released during the differentiation of colorectal cancer stem cells. Here we show that sEVs secretion during colon cancer stem cells differentiation is partially controlled by CD147, a well-known player involved in colon cancer tumorigenesis. CD147 + sEVs activate a signaling cascade in recipient cells inducing molecular invasive features in colon cancer cells. CD147 knockdown as well as anti-CD147 antibodies impaired sEVs release and downstream effects on recipient cells and blocking multivesicular body maturation prevented sEVs release during the differentiation. Our findings reveal a functional role of CD147 in promoting sEVs release during the differentiation of colon cancer stem cells and in triggering cellular changes in recipient cells.
KW - CD147
KW - colorectal cancer stem cells
KW - differentiation
KW - small extracellular vesicles
KW - CD147
KW - colorectal cancer stem cells
KW - differentiation
KW - small extracellular vesicles
UR - http://hdl.handle.net/10807/154761
U2 - 10.3390/cancers12020260
DO - 10.3390/cancers12020260
M3 - Article
SN - 2072-6694
VL - 2020/12
SP - 260-N/A
JO - Cancers
JF - Cancers
ER -
