CD133+ stem cell mobilization after partial hepatectomy depends on resection extent and underlying disease

Maria Assunta Zocco, Felice Giuliante, Vincenzo Arena, Gennaro Nuzzo, Antonio Gasbarrini, Marialuisa Novi, Emanuele Rinninella, Annalisa Tortora, Carlo Rumi, Gabriele Bombardieri, Ac Piscaglia, Fm Vecchio

Risultato della ricerca: Contributo in rivistaArticolo in rivista

10 Citazioni (Scopus)

Abstract

BACKGROUND: Bone marrow stem cells (BMSC) can participate to liver regeneration. However, conflicting results have been reported on this topic in patients undergoing liver resection. AIMS: To assess the impact of liver resection extent and presence of underlying liver disease in modulating BMSC mobilization. METHODS: We enrolled 29 patients undergoing liver resection of different extents, 5 surgical controls and 10 blood donors. Circulating CD133+ BMSC were measured by flow cytometry at different time-points after surgery. The hepatic commitment of mobilized BMSC was investigated by polymerase chain reaction. Liver specimens were collected during surgery for histopathological analysis. Hepatocyte growth factor and granulocyte-colony stimulating factor serum levels were measured by enzyme-linked immunosorbent assay. RESULTS: BMSC mobilization was found in patients undergoing major liver resection, especially in the presence of underlying disease. Ductular reactions were noted in patients with chronic hepatopathy and the hepatic progenitor-like cells expressed CD133, NCAM, cytokeratin-19, and alpha-fetoprotein. Hepatocyte growth factor and granulocyte-colony stimulating factor levels increased following liver resection and the contemporaneous presence of liver disease was associated with their highest raise. CONCLUSIONS: Liver repair is mainly an endogenous process. BMSC become important in case of extensive resection, especially in the presence of underlying hepatopathy and hepatic progenitor-like cells activation. Hepatocyte growth factor and granulocyte-colony stimulating factor seem to be involved in the dynamics underlying hepatic regeneration and BMSC recruitment.
Lingua originaleEnglish
pagine (da-a)147-154
Numero di pagine8
RivistaDIGESTIVE AND LIVER DISEASE
Volume43
DOI
Stato di pubblicazionePubblicato - 2011

Keywords

  • Antigens, CD
  • Biological Markers
  • Case-Control Studies
  • Female
  • Glycoproteins
  • Granulocyte Colony-Stimulating Factor
  • Hematopoietic Stem Cell Mobilization
  • Hepatectomy
  • Hepatocyte Growth Factor
  • Humans
  • Keratin-19
  • Liver Diseases
  • Liver Regeneration
  • Male
  • Middle Aged
  • Neural Cell Adhesion Molecules
  • Peptides
  • alpha-Fetoproteins

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