Abstract
BACKGROUND:
Bone marrow stem cells (BMSC) can participate to liver regeneration. However, conflicting results have been reported on this topic in patients undergoing liver resection.
AIMS:
To assess the impact of liver resection extent and presence of underlying liver disease in modulating BMSC mobilization.
METHODS:
We enrolled 29 patients undergoing liver resection of different extents, 5 surgical controls and 10 blood donors. Circulating CD133+ BMSC were measured by flow cytometry at different time-points after surgery. The hepatic commitment of mobilized BMSC was investigated by polymerase chain reaction. Liver specimens were collected during surgery for histopathological analysis. Hepatocyte growth factor and granulocyte-colony stimulating factor serum levels were measured by enzyme-linked immunosorbent assay.
RESULTS:
BMSC mobilization was found in patients undergoing major liver resection, especially in the presence of underlying disease. Ductular reactions were noted in patients with chronic hepatopathy and the hepatic progenitor-like cells expressed CD133, NCAM, cytokeratin-19, and alpha-fetoprotein. Hepatocyte growth factor and granulocyte-colony stimulating factor levels increased following liver resection and the contemporaneous presence of liver disease was associated with their highest raise.
CONCLUSIONS:
Liver repair is mainly an endogenous process. BMSC become important in case of extensive resection, especially in the presence of underlying hepatopathy and hepatic progenitor-like cells activation. Hepatocyte growth factor and granulocyte-colony stimulating factor seem to be involved in the dynamics underlying hepatic regeneration and BMSC recruitment.
Lingua originale | English |
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pagine (da-a) | 147-154 |
Numero di pagine | 8 |
Rivista | Digestive and Liver Disease |
Volume | 43 |
DOI | |
Stato di pubblicazione | Pubblicato - 2011 |
Keywords
- Antigens, CD
- Biological Markers
- Case-Control Studies
- Female
- Glycoproteins
- Granulocyte Colony-Stimulating Factor
- Hematopoietic Stem Cell Mobilization
- Hepatectomy
- Hepatocyte Growth Factor
- Humans
- Keratin-19
- Liver Diseases
- Liver Regeneration
- Male
- Middle Aged
- Neural Cell Adhesion Molecules
- Peptides
- alpha-Fetoproteins