Caspase-8 dependent apoptosis induction in malignant myeloid cells by TLR stimulation in the presence of IFN-alpha

Ornella Parolini, Manfred Lehner, Marco Bailo, Daniel Stachel, Wolf Roesler, Wolfgang Holter

Risultato della ricerca: Contributo in rivistaArticolo in rivista

15 Citazioni (Scopus)

Abstract

[Ita:]Pro-apoptotic signalling upon toll-like receptor (TLR) stimulation in myeloid cells is normally antagonized by the simultaneous activation of anti-apoptotic pathways. We have previously reported that IFN-alpha can sensitize human monocytes to apoptosis induction by lipopolysaccharide (LPS). Based on these results we investigated whether similarly apoptosis can be cooperatively induced in myeloid tumor cells. When testing established acute myeloid leukemia (AML) cell lines we found the monocytic cell line THP-1 to be sensitive to IFN-alpha plus LPS induced apoptosis, which was partially dependent on caspase-8 and was associated with an enhanced expression of Fas/CD95. We extended our study to 29 short term blast lines from patients with AML and observed additive effects of IFN-alpha and LPS on cell death only with few samples indicating that sensitivity to IFN-alpha plus LPS inducible apoptosis is present in a fraction of AML samples only with no obvious correlation with certain FAB phenotypes
Lingua originaleEnglish
pagine (da-a)1729-1735
Numero di pagine7
RivistaLeukemia Research
Volume31
DOI
Stato di pubblicazionePubblicato - 2007

Keywords

  • Apoptosis
  • Caspase 8
  • Cell Line
  • Cell Line, Tumor
  • Humans
  • Interferon-alpha
  • Leukemia, Myeloid, Acute
  • Lipopolysaccharides
  • Monocytes
  • Myeloid Cells
  • Toll-Like Receptors

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