TY - JOUR
T1 - Brain invasion along perivascular spaces by glioma cells: Relationship with blood–brain barrier
AU - Pacioni, S.
AU - D'Alessandris, Quintino Giorgio
AU - Buccarelli, M.
AU - Boe, A.
AU - Martini, M.
AU - Larocca, L. M.
AU - Bolasco, G.
AU - Ricci-Vitiani, L.
AU - Falchetti, M. L.
AU - Pallini, R.
PY - 2020
Y1 - 2020
N2 - The question whether perivascular glioma cells invading the brain far from the tumor bulk may disrupt the blood–brain barrier (BBB) represents a crucial issue because under this condition tumor cells would be no more protected from the reach of chemotherapeutic drugs. A recent in vivo study that used human xenolines, demonstrated that single glioma cells migrating away from the tumor bulk are sufficient to breach the BBB. Here, we used brain xenografts of patient-derived glioma stem-like cells (GSCs) to show by immunostaining that in spite of massive perivascular invasion, BBB integrity was preserved in the majority of vessels located outside the tumor bulk. Interestingly, the tumor cells that invaded the brain for the longest distances traveled along vessels with retained BBB integrity. In surgical specimens of malignant glioma, the area of brain invasion showed several vessels with preserved BBB that were surrounded by tumor cells. On transmission electron microscopy, the cell inter-junctions and basal lamina of the brain endothelium were preserved even in conditions in which the tumor cells lay adjacently to blood vessels. In conclusion, BBB integrity associates with extensive perivascular invasion of glioma cells.
AB - The question whether perivascular glioma cells invading the brain far from the tumor bulk may disrupt the blood–brain barrier (BBB) represents a crucial issue because under this condition tumor cells would be no more protected from the reach of chemotherapeutic drugs. A recent in vivo study that used human xenolines, demonstrated that single glioma cells migrating away from the tumor bulk are sufficient to breach the BBB. Here, we used brain xenografts of patient-derived glioma stem-like cells (GSCs) to show by immunostaining that in spite of massive perivascular invasion, BBB integrity was preserved in the majority of vessels located outside the tumor bulk. Interestingly, the tumor cells that invaded the brain for the longest distances traveled along vessels with retained BBB integrity. In surgical specimens of malignant glioma, the area of brain invasion showed several vessels with preserved BBB that were surrounded by tumor cells. On transmission electron microscopy, the cell inter-junctions and basal lamina of the brain endothelium were preserved even in conditions in which the tumor cells lay adjacently to blood vessels. In conclusion, BBB integrity associates with extensive perivascular invasion of glioma cells.
KW - Blood-Brain barrier
KW - Brain endothelium
KW - Glioblastoma
KW - Glioma stem-like cells
KW - Perivascular invasion
KW - Blood-Brain barrier
KW - Brain endothelium
KW - Glioblastoma
KW - Glioma stem-like cells
KW - Perivascular invasion
UR - https://publicatt.unicatt.it/handle/10807/151096
UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85077180817&origin=inward
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85077180817&origin=inward
U2 - 10.3390/cancers12010018
DO - 10.3390/cancers12010018
M3 - Article
SN - 2072-6694
VL - 12
SP - 18-N/A
JO - Cancers
JF - Cancers
IS - 1
ER -