Bmi1 is expressed in vivo in intestinal stem cells

Eugenio Sangiorgi, Mario R. Capecchi

Risultato della ricerca: Contributo in rivistaArticolo in rivista

878 Citazioni (Scopus)

Abstract

Bmi1 plays an essential part in the self-renewal of hematopoietic and neural stem cells. To investigate its role in other adult stem cell populations, we generated a mouse expressing a tamoxifen-inducible Cre from the Bmi1 locus. We found that Bmi1 is expressed in discrete cells located near the bottom of crypts in the small intestine, predominantly four cells above the base of the crypt (+4 position). Over time, these cells proliferate, expand, self-renew and give rise to all the differentiated cell lineages of the small intestine epithelium. The induction of a stable form of beta-catenin in these cells was sufficient to rapidly generate adenomas. Moreover, ablation of Bmi1(+) cells using a Rosa26 conditional allele, expressing diphtheria toxin, led to crypt loss. These experiments identify Bmi1 as an intestinal stem cell marker in vivo. Unexpectedly, the distribution of Bmi1-expressing stem cells along the length of the small intestine suggested that mammals use more than one molecularly distinguishable adult stem cell subpopulation to maintain organ homeostasis.
Lingua originaleEnglish
pagine (da-a)915-920
Numero di pagine6
RivistaNature Genetics
Volume40
DOI
Stato di pubblicazionePubblicato - 2008

Keywords

  • Adult Stem Cells
  • Animals
  • Homeostasis
  • Intestines
  • Mice
  • Mice, Transgenic
  • Nuclear Proteins
  • Proto-Oncogene Proteins
  • Repressor Proteins
  • Tissue Distribution

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