The nanomechanical properties of SW480 colon cancer cells were investigated using Atomic Force
Microscopy. SW480 cells are composed of two sub-populations with different shape and invasiveness.
These two cells populations showed similar adhesion properties while appeared significantly
different in term of cells stiffness. Since cell stiffness is related to invasiveness and growth, we suggest
elasticity as a useful parameter to distinguish invasive cells inside the colorectal tumor bulk
and the high-resolution mechanical mapping as a promising diagnostic tool for the identification of