TY - JOUR
T1 - Bacteria of the genus Asaia stably associate with Anopheles stephensi, an Asian malarial mosquito vector
AU - Favia, Guido
AU - Ricci, Irene
AU - Damiani, Claudia
AU - Raddadi, Noura
AU - Crotti, Elena
AU - Marzorati, Massimo
AU - Rizzi, Aurora
AU - Urso, Roberta
AU - Brusetti, Lorenzo
AU - Borin, Sara
AU - Mora, Diego
AU - Scuppa, Patrizia
AU - Pasqualini, Luciano
AU - Clementi, Emanuela
AU - Genchi, Marco
AU - Corona, Silvia
AU - Negri, Ilaria
AU - Grandi, Giulio
AU - Alma, Alberto
AU - Kramer, Laura
AU - Esposito, Fulvio
AU - Bandi, Claudio
AU - Sacchi, Luciano
AU - Daffonchio, Daniele
PY - 2007
Y1 - 2007
N2 - Here, we show that an alpha-proteobacterium of the genus Asaia is stably associated with larvae and adults of Anopheles stephensi, an important mosquito vector of Plasmodium vivax, a main malaria agent in Asia. Asaia bacteria dominate mosquito-associated microbiota, as shown by 16S rRNA gene abundance, quantitative PCR, transmission electron microscopy and in situ-hybridization of 16S rRNA genes. In adult mosquitoes, Asaia sp. is present in high population density in the female gut and in the male reproductive tract. Asaia sp. from An. stephensi has been cultured in cell-free media and then transformed with foreign DNA. A green fluorescent protein-tagged Asaia sp. strain effectively lodged in the female gut and salivary glands, sites that are crucial for Plasmodium sp. development and transmission. The larval gut and the male reproductive system were also colonized by the transformed Asaia sp. strain. As an efficient inducible colonizer of mosquitoes that transmit Plasmodium sp., Asaia sp. may be a candidate for malaria control.
AB - Here, we show that an alpha-proteobacterium of the genus Asaia is stably associated with larvae and adults of Anopheles stephensi, an important mosquito vector of Plasmodium vivax, a main malaria agent in Asia. Asaia bacteria dominate mosquito-associated microbiota, as shown by 16S rRNA gene abundance, quantitative PCR, transmission electron microscopy and in situ-hybridization of 16S rRNA genes. In adult mosquitoes, Asaia sp. is present in high population density in the female gut and in the male reproductive tract. Asaia sp. from An. stephensi has been cultured in cell-free media and then transformed with foreign DNA. A green fluorescent protein-tagged Asaia sp. strain effectively lodged in the female gut and salivary glands, sites that are crucial for Plasmodium sp. development and transmission. The larval gut and the male reproductive system were also colonized by the transformed Asaia sp. strain. As an efficient inducible colonizer of mosquitoes that transmit Plasmodium sp., Asaia sp. may be a candidate for malaria control.
KW - insect vector, anopheles
KW - insect vector, anopheles
UR - http://hdl.handle.net/10807/105657
U2 - 10.1073/pnas.0610451104
DO - 10.1073/pnas.0610451104
M3 - Article
SN - 0027-8424
VL - 104
SP - 9047
EP - 9051
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
ER -