ATP released by intestinal bacteria limits the generation of protective IgA against enteropathogens

Michele Proietti, Lisa Perruzza, Daniela Scribano, Giovanni Pellegrini, Rocco D’Antuono, Francesco Strati, Marco Raffaelli, Santiago F. Gonzalez, Marcus Thelen, Wolf-Dietrich Hardt, Emma Slack, Mauro Nicoletti, Fabio Grassi

Risultato della ricerca: Contributo in rivistaArticolo in rivista

22 Citazioni (Scopus)


T cell dependent secretory IgA (SIgA) generated in the Peyer’s patches (PPs) of the small intestine shapes a broadly diverse microbiota that is crucial for host physiology. The mutualistic co-evolution of host and microbes led to the relative tolerance of host’s immune system towards commensal microorganisms. The ATP-gated ionotropic P2X7 receptor limits T follicular helper (Tfh) cells expansion and germinal center (GC) reaction in the PPs. Here we show that transient depletion of intestinal ATP can dramatically improve high-affinity IgA response against both live and inactivated oral vaccines. Ectopic expression of Shigella flexneri periplasmic ATP-diphosphohydrolase (apyrase) abolishes ATP release by bacteria and improves the specific IgA response against live oral vaccines. Antibody responses primed in the absence of intestinal extracellular ATP (eATP) also provide superior protection from enteropathogenic infection. Thus, modulation of eATP in the small intestine can affect high-affinity IgA response against gut colonizing bacteria.
Lingua originaleEnglish
pagine (da-a)250-261
Numero di pagine12
RivistaNature Communications
Stato di pubblicazionePubblicato - 2019


  • Adenosine Triphosphate
  • Administration, Oral
  • Animals
  • Apyrase
  • Bacterial Proteins
  • Bacterial Vaccines
  • Biochemistry, Genetics and Molecular Biology (all)
  • Chemistry (all)
  • Disease Models, Animal
  • Escherichia coli
  • Female
  • Gastroenteritis
  • Gastrointestinal Microbiome
  • Germinal Center
  • Humans
  • Ileum
  • Immunoglobulin A, Secretory
  • Intestinal Mucosa
  • Mice
  • Mice, Inbred C57BL
  • Peyer's Patches
  • Physics and Astronomy (all)
  • Receptors, Purinergic P2X7
  • Salmonella Infections
  • Salmonella typhimurium
  • Shigella flexneri
  • T-Lymphocytes, Helper-Inducer


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