Association of human leucocyte antigen loci with vaccine-induced immune thrombotic thrombocytopenia: Potential role of the interaction between platelet factor 4-derived peptides and MHC-II

Eleonora Petito; Loredana Bury; Lilian Antunes Heck; Brooke Sadler; Erica De Candia; Gian Marco Podda; Anna Falanga; Lucia Stefanini; Andrea Boccatonda; Patrizia Sciancalepore; Igor Florio; Egidio Imbalzano; Rossella Marcucci; Patrizia Noris; Marta Panella; Rita Carlotta Santoro; Maria Costanza Turi; Gaetano Vaudo; Jorge Di Paola; Matthew T Rondina; Paolo Gresele

doi:10.1111/bjh.19838

Association of human leucocyte antigen loci with vaccine-induced immune thrombotic thrombocytopenia: Potential role of the interaction between platelet factor 4-derived peptides and MHC-II

Eleonora Petito
, Loredana Bury
, Lilian Antunes Heck
, Brooke Sadler
, Erica De Candia
, Gian Marco Podda
, Anna Falanga
, Lucia Stefanini
, Andrea Boccatonda
, Patrizia Sciancalepore
, Igor Florio
, Egidio Imbalzano
, Rossella Marcucci
, Patrizia Noris
, Marta Panella
, Rita Carlotta Santoro
, Maria Costanza Turi
, Gaetano Vaudo
, Jorge Di Paola
, Matthew T Rondina

Paolo Gresele^*

^*Autore corrispondente per questo lavoro

University of Perugia
Washington University St. Louis
University of Milan
ASST Papa Giovanni XXIII
University “La Sapienza” of Rome
Azienda USL di Bologna
Azienda Ospedaliera Nazionale Santi Antonio e Biagio e Cesare Arrigo
University of Messina
University of Florence
IRCCS Fondazione Policlinico San Matteo - Pavia
University of Pavia
University of Rome Tor Vergata
Azienda Ospedaliera Pugliese-Ciaccio

Risultato della ricerca: Contributo in rivista › Articolo

Abstract

No risk factors have been identified for vaccine-induced immune thrombotic thrombocytopenia (VITT) so far. The aim of this study was to identify human leucocyte antigen (HLA) alleles potentially associated with VITT susceptibility. Specific HLA class II alleles were detected with significantly higher frequency in VITT patients compared with Italian controls: DPB1*17:01, DQA1*05:01, and DRB1*11:04. In silico analysis revealed increased affinity of DRB1*11:04 for a platelet factor 4 (PF4)-derived peptide, ITSLEVIKA, that contains two amino acids present in the specific binding site of anti-PF4 antibodies from VITT patients. Our findings show for the first time a genetic predisposition to developing anti-PF4 antibodies in response to Ad-vector vaccines.

Lingua originale	Inglese
pagine (da-a)	290-295
Numero di pagine	6
Rivista	British Journal of Haematology
Volume	206
Numero di pubblicazione	1
DOI	https://doi.org/10.1111/bjh.19838
Stato di pubblicazione	Pubblicato - 2025

OSS delle Nazioni Unite

Questo processo contribuisce al raggiungimento dei seguenti obiettivi di sviluppo sostenibile

SDG 3 Salute e benessere

All Science Journal Classification (ASJC) codes

Ematologia

Keywords

HLA
PF4
VITT

Accesso al documento

10.1111/bjh.19838

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Petito, E., Bury, L., Antunes Heck, L., Sadler, B., De Candia, E., Podda, G. M., Falanga, A., Stefanini, L., Boccatonda, A., Sciancalepore, P., Florio, I., Imbalzano, E., Marcucci, R., Noris, P., Panella, M., Santoro, R. C., Turi, M. C., Vaudo, G., Di Paola, J., ... Gresele, P. (2025). Association of human leucocyte antigen loci with vaccine-induced immune thrombotic thrombocytopenia: Potential role of the interaction between platelet factor 4-derived peptides and MHC-II. British Journal of Haematology, 206(1), 290-295. https://doi.org/10.1111/bjh.19838

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title = "Association of human leucocyte antigen loci with vaccine-induced immune thrombotic thrombocytopenia: Potential role of the interaction between platelet factor 4-derived peptides and MHC-II",

abstract = "No risk factors have been identified for vaccine-induced immune thrombotic thrombocytopenia (VITT) so far. The aim of this study was to identify human leucocyte antigen (HLA) alleles potentially associated with VITT susceptibility. Specific HLA class II alleles were detected with significantly higher frequency in VITT patients compared with Italian controls: DPB1*17:01, DQA1*05:01, and DRB1*11:04. In silico analysis revealed increased affinity of DRB1*11:04 for a platelet factor 4 (PF4)-derived peptide, ITSLEVIKA, that contains two amino acids present in the specific binding site of anti-PF4 antibodies from VITT patients. Our findings show for the first time a genetic predisposition to developing anti-PF4 antibodies in response to Ad-vector vaccines.",

keywords = "HLA, PF4, VITT, HLA, PF4, VITT",

author = "Eleonora Petito and Loredana Bury and \{Antunes Heck\}, Lilian and Brooke Sadler and \{De Candia\}, Erica and Podda, \{Gian Marco\} and Anna Falanga and Lucia Stefanini and Andrea Boccatonda and Patrizia Sciancalepore and Igor Florio and Egidio Imbalzano and Rossella Marcucci and Patrizia Noris and Marta Panella and Santoro, \{Rita Carlotta\} and Turi, \{Maria Costanza\} and Gaetano Vaudo and \{Di Paola\}, Jorge and Rondina, \{Matthew T\} and Paolo Gresele",

year = "2025",

doi = "10.1111/bjh.19838",

language = "English",

volume = "206",

pages = "290--295",

journal = "British Journal of Haematology",

issn = "1365-2141",

publisher = "John Wiley and Sons Inc.",

number = "1",

}

Petito, E, Bury, L, Antunes Heck, L, Sadler, B, De Candia, E, Podda, GM, Falanga, A, Stefanini, L, Boccatonda, A, Sciancalepore, P, Florio, I, Imbalzano, E, Marcucci, R, Noris, P, Panella, M, Santoro, RC, Turi, MC, Vaudo, G, Di Paola, J, Rondina, MT & Gresele, P 2025, 'Association of human leucocyte antigen loci with vaccine-induced immune thrombotic thrombocytopenia: Potential role of the interaction between platelet factor 4-derived peptides and MHC-II', British Journal of Haematology, vol. 206, n. 1, pagg. 290-295. https://doi.org/10.1111/bjh.19838

Association of human leucocyte antigen loci with vaccine-induced immune thrombotic thrombocytopenia: Potential role of the interaction between platelet factor 4-derived peptides and MHC-II. / Petito, Eleonora; Bury, Loredana; Antunes Heck, Lilian et al.
In: British Journal of Haematology, Vol. 206, N. 1, 2025, pag. 290-295.

Risultato della ricerca: Contributo in rivista › Articolo

TY - JOUR

T1 - Association of human leucocyte antigen loci with vaccine-induced immune thrombotic thrombocytopenia: Potential role of the interaction between platelet factor 4-derived peptides and MHC-II

AU - Petito, Eleonora

AU - Bury, Loredana

AU - Antunes Heck, Lilian

AU - Sadler, Brooke

AU - De Candia, Erica

AU - Podda, Gian Marco

AU - Falanga, Anna

AU - Stefanini, Lucia

AU - Boccatonda, Andrea

AU - Sciancalepore, Patrizia

AU - Florio, Igor

AU - Imbalzano, Egidio

AU - Marcucci, Rossella

AU - Noris, Patrizia

AU - Panella, Marta

AU - Santoro, Rita Carlotta

AU - Turi, Maria Costanza

AU - Vaudo, Gaetano

AU - Di Paola, Jorge

AU - Rondina, Matthew T

AU - Gresele, Paolo

PY - 2025

Y1 - 2025

N2 - No risk factors have been identified for vaccine-induced immune thrombotic thrombocytopenia (VITT) so far. The aim of this study was to identify human leucocyte antigen (HLA) alleles potentially associated with VITT susceptibility. Specific HLA class II alleles were detected with significantly higher frequency in VITT patients compared with Italian controls: DPB1*17:01, DQA1*05:01, and DRB1*11:04. In silico analysis revealed increased affinity of DRB1*11:04 for a platelet factor 4 (PF4)-derived peptide, ITSLEVIKA, that contains two amino acids present in the specific binding site of anti-PF4 antibodies from VITT patients. Our findings show for the first time a genetic predisposition to developing anti-PF4 antibodies in response to Ad-vector vaccines.

AB - No risk factors have been identified for vaccine-induced immune thrombotic thrombocytopenia (VITT) so far. The aim of this study was to identify human leucocyte antigen (HLA) alleles potentially associated with VITT susceptibility. Specific HLA class II alleles were detected with significantly higher frequency in VITT patients compared with Italian controls: DPB1*17:01, DQA1*05:01, and DRB1*11:04. In silico analysis revealed increased affinity of DRB1*11:04 for a platelet factor 4 (PF4)-derived peptide, ITSLEVIKA, that contains two amino acids present in the specific binding site of anti-PF4 antibodies from VITT patients. Our findings show for the first time a genetic predisposition to developing anti-PF4 antibodies in response to Ad-vector vaccines.

KW - HLA

KW - PF4

KW - VITT

KW - HLA

KW - PF4

KW - VITT

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U2 - 10.1111/bjh.19838

DO - 10.1111/bjh.19838

M3 - Article

SN - 1365-2141

VL - 206

SP - 290

EP - 295

JO - British Journal of Haematology

JF - British Journal of Haematology

IS - 1

ER -

Association of human leucocyte antigen loci with vaccine-induced immune thrombotic thrombocytopenia: Potential role of the interaction between platelet factor 4-derived peptides and MHC-II

Abstract

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All Science Journal Classification (ASJC) codes

Keywords

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