BACKGROUND: Candida mannan (Mn) detection in bronchoalveolar lavage fluid (BALF)
was shown to be useful for earlier identification and preemptive therapy
targeting in preterm infants at high risk of invasive Candida infection. We
investigated whether early detection of Candida Mn in BALF is associated with the
presence of some neutrophilic products, as markers of prenatal
METHODS: BALF specimens were collected during the first 48 h of life from
mechanically ventilated preterm newborns. Samples were analyzed by
high-performance liquid chromatography-electrospray ionization-mass spectrometry.
The relative amounts of α-defensins 1-4 and S100A proteins were measured by
extracted ion current peak area. Absolute and differential white cell counts in
BALF were obtained. Mn antigen concentrations were determined by the Platelia
Candida antigen kit.
RESULTS: Twenty-five studied neonates were divided into two groups: Mn-positive
group and Mn-negative group. Levels of α-defensins 1-4 and S100A12 were
significantly higher in the Mn-positive group than in the Mn-negative group.
Moreover, positive significant correlations between the absolute number of
neutrophils and the levels of α-defensins 1-4 and S100A8 were observed.
CONCLUSION: The detection of Mn antigen in BALF of preterm infants is consistent
with evidence of an innate immune response in their lungs as demonstrated by
higher levels of α-defensins and S100A proteins.
- Bronchoalveolar Lavage Fluid
- Chromatography, High Pressure Liquid
- Infant, Newborn
- Infant, Premature
- S100 Proteins
- Spectrometry, Mass, Electrospray Ionization