TY - JOUR
T1 - Antioxidant and anti-inflammatory effects of selected natural compounds contained
in a dietary supplement on two human immortalized keratinocyte lines
AU - Serini, Simona
AU - Calviello, Gabriella
AU - Fasano, Elena
AU - Celleno, Leonardo
AU - Lanza, Paola
AU - Cittadini, Achille Renato Maria
AU - Mondella, Nadia
AU - Trombino, Sonia
PY - 2014
Y1 - 2014
N2 - Several advantages may derive from the use of dietary supplements containing
multiple natural antioxidants and/or anti-inflammatory agents. At present,
however, there is scarce information on the properties and potential of combined
supplements. To fill the gap, the antioxidant and anti-inflammatory activities
exerted by a combination of seven natural components (coenzyme Q10, krill oil,
lipoic acid, resveratrol, grape seed oil, α-tocopherol, and selenium) contained
in a dietary supplement used for the prevention of skin disorders were
investigated in vitro. Each component was administered, alone or in combination,
to human keratinocytes, and the inhibition of Reactive Oxygen Species production
and lipid peroxidation as well as the ability to reduce inflammatory cytokine
secretion and to modulate Nuclear Factor-κB pathway was evaluated. The
combination exhibited high antioxidant activity and in specific conditions the
combination's efficiency was higher than that of the most powerful components
administered individually. Moreover, the combination showed remarkable
anti-inflammatory properties. It reduced more efficiently than each component the
secretion of Monocyte Chemoattractant Protein-1, a crucial cytokine for the
development of chronic inflammation in skin, and inhibited Nuclear Factor-κB
molecular pathway. Overall, our findings suggest that the combined formulation
may have the potential to powerfully inhibit oxidative stress and inflammation at
skin level.
AB - Several advantages may derive from the use of dietary supplements containing
multiple natural antioxidants and/or anti-inflammatory agents. At present,
however, there is scarce information on the properties and potential of combined
supplements. To fill the gap, the antioxidant and anti-inflammatory activities
exerted by a combination of seven natural components (coenzyme Q10, krill oil,
lipoic acid, resveratrol, grape seed oil, α-tocopherol, and selenium) contained
in a dietary supplement used for the prevention of skin disorders were
investigated in vitro. Each component was administered, alone or in combination,
to human keratinocytes, and the inhibition of Reactive Oxygen Species production
and lipid peroxidation as well as the ability to reduce inflammatory cytokine
secretion and to modulate Nuclear Factor-κB pathway was evaluated. The
combination exhibited high antioxidant activity and in specific conditions the
combination's efficiency was higher than that of the most powerful components
administered individually. Moreover, the combination showed remarkable
anti-inflammatory properties. It reduced more efficiently than each component the
secretion of Monocyte Chemoattractant Protein-1, a crucial cytokine for the
development of chronic inflammation in skin, and inhibited Nuclear Factor-κB
molecular pathway. Overall, our findings suggest that the combined formulation
may have the potential to powerfully inhibit oxidative stress and inflammation at
skin level.
KW - anti-inflammatory
KW - antioxidant
KW - cytokines
KW - keratinocytes
KW - anti-inflammatory
KW - antioxidant
KW - cytokines
KW - keratinocytes
UR - http://hdl.handle.net/10807/60277
U2 - 10.1155/2014/327452
DO - 10.1155/2014/327452
M3 - Article
SP - 1
EP - 11
JO - BioMed Research International
JF - BioMed Research International
SN - 2314-6133
ER -