TY - JOUR
T1 - Antimicrobial and antibiofilm properties of graphene oxide on enterococcus faecalis
AU - Martini, Cecilia
AU - Longo, Fabio
AU - Castagnola, Raffaella
AU - Marigo, Luca
AU - Grande, Nicola Maria
AU - Cordaro, Massimo
AU - Cacaci, Margherita
AU - Papi, Massimiliano
AU - Palmieri, Valentina
AU - Bugli, Francesca
AU - Sanguinetti, Maurizio
PY - 2020
Y1 - 2020
N2 - The aim of this study was to evaluate the antibacterial properties of graphene oxide (GO) against Enterococcus faecalis in vitro conditions and when used to coat dentin surface to prevent E. faecalis adhesion. The ATCC strain of E. faecalis 29212 has been used to perform a viability test. The pellet was suspended in ultrapure water, NaCl, PBS buffer, CaCl2 and MgCl2, Luria−Bertani broth solutions. The viability was evaluated by the colony forming unit counting method. Atomic force microscopy images and the measure of surface zeta potential variation were analyzed. Dentin discs were covered with a film of GO (n = 15) or were not treated (n = 15). Bacterial suspension was added to each sample of dentine discs and microbial counts were calculated. Statistically significant differences between two groups were assessed by a two‐tailed unpaired t‐test. Bacteria cell morphology was investigated with scanning electron microscopy. The highest growth inhibition was obtained in ddH2O and CaCl2 solution while, in PBS and NaCl, GO had poor antibacterial efficacy with a growth enhancing effect in the latter. GO on dentin discs demonstrated high antibacterial activity. GO film has demonstrated acceptable adhesion properties to root dentin and a role in the inhibition of bacterial film proliferation and biofilm formation.
AB - The aim of this study was to evaluate the antibacterial properties of graphene oxide (GO) against Enterococcus faecalis in vitro conditions and when used to coat dentin surface to prevent E. faecalis adhesion. The ATCC strain of E. faecalis 29212 has been used to perform a viability test. The pellet was suspended in ultrapure water, NaCl, PBS buffer, CaCl2 and MgCl2, Luria−Bertani broth solutions. The viability was evaluated by the colony forming unit counting method. Atomic force microscopy images and the measure of surface zeta potential variation were analyzed. Dentin discs were covered with a film of GO (n = 15) or were not treated (n = 15). Bacterial suspension was added to each sample of dentine discs and microbial counts were calculated. Statistically significant differences between two groups were assessed by a two‐tailed unpaired t‐test. Bacteria cell morphology was investigated with scanning electron microscopy. The highest growth inhibition was obtained in ddH2O and CaCl2 solution while, in PBS and NaCl, GO had poor antibacterial efficacy with a growth enhancing effect in the latter. GO on dentin discs demonstrated high antibacterial activity. GO film has demonstrated acceptable adhesion properties to root dentin and a role in the inhibition of bacterial film proliferation and biofilm formation.
KW - Antimicrobial properties
KW - Biofilm
KW - Enterococcus faecalis
KW - Graphene oxide
KW - Radicular dentin
KW - Antimicrobial properties
KW - Biofilm
KW - Enterococcus faecalis
KW - Graphene oxide
KW - Radicular dentin
UR - http://hdl.handle.net/10807/162245
U2 - 10.3390/antibiotics9100692
DO - 10.3390/antibiotics9100692
M3 - Article
SN - 2079-6382
VL - 9
SP - 1
EP - 11
JO - Antibiotics
JF - Antibiotics
ER -