Analysis of the hippo transducers TAZ and YAP in cervical cancer and its microenvironment

Ruggero De Maria Marchiano, Teresa Gamucci, Ilio Vitale, Simonetta Buglioni, Patrizia Vici, Domenico Sergi, Laura Pizzuti, Luigi Di Lauro, Barbara Antoniani, Francesca Sperati, Irene Terrenato, Mariantonia Carosi, Cristina Vincenzoni, Luciano Mariani, Enrico Vizza, Aldo Venuti, Giuseppe Sanguineti, Angiolo Gadducci, Maddalena Barba, Clara NatoliMarcella Mottolese, Marcello Maugeri-Saccà

Risultato della ricerca: Contributo in rivistaArticolo in rivista

21 Citazioni (Scopus)

Abstract

Hippo is a tumor-suppressor pathway that negatively regulates the oncoproteins TAZ and YAP. Moreover, Hippo affects the biology of a variety of non-neoplastic cells in the tumor microenvironment, even including immune cells. We herein assessed the predictive role of TAZ and YAP, assessed by immunohistochemistry, in 50 cervical cancer patients prevalently treated with neoadjuvant chemotherapy. Tumors were classified as positive or negative according to the percentage of tumor-expressing cells and cellular localization. TAZ/YAP were also evaluated in non-neoplastic cells, namely endothelial cells, non-lymphocytic stromal cells and tumor-infiltrating lymphocytes (TILs). TAZ expression in cancer cells (TAZpos) was associated with a reduced pathological complete response (pCR) rate (p = 0.041). Conversely, the expression of TAZ and YAP in TILs (TAZTIL+and YAPTIL+) seemed to be associated with increased pCRs (p = 0.083 and p = 0.018, respectively). When testing the predictive significance of the concomitant expression of TAZ in cancer cells and its absence in TILs (TAZpos/TAZTIL-), patients with TAZpos/TAZTIL-showed lower pCR rate (p = 0.001), as confirmed in multivariate analysis (TAZpos/TAZTIL-: OR 8.67, 95% CI: 2.31–32.52, p = 0.001). Sensitivity analysis carried out in the 41 patients treated with neoadjuvant chemotherapy yielded comparable results (TAZpos/TAZTIL-: OR 11.0, 95% CI: 2.42–49.91, p = 0.002). Internal validation carried out with two different procedures confirmed the robustness of this model. Overall, we found evidence on the association between TAZ expression in cervical cancer cells and reduced pCR rate. Conversely, the expression of the Hippo transducers in TILs may predict increased treatment efficacy, possibly mirroring the activation of a non-canonical Hippo/MST pathway necessary for T-cells activation and survival.
Lingua originaleEnglish
pagine (da-a)N/A-N/A
RivistaOncoImmunology
Volume5
DOI
Stato di pubblicazionePubblicato - 2016

Keywords

  • Cervical cancer
  • Hippo pathway
  • Hippo transducers
  • Immunology
  • Immunology and Allergy
  • Oncology
  • TAZ
  • YAP
  • pathological complete response
  • tumor microenvironment

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