Analysis of the cross-talk between maize and Fusarium verticillioides revealed by RNA-Sequencing.

Alessandra Lanubile, Adriano Marocco, Valentina Maschietto, Irene Falasconi, Alberto Ferrarini, Diana Bellin, Massimo Delledonne

Risultato della ricerca: Contributo in libroContributo a convegno

Abstract

Fusarium verticillioides is a plant pathogen able to produce fumonisin in maize kernels. To clarify the molecular processes undergoing in maize upon infection, the expression profiles of resistant and susceptible genotypes after F. verticillioides inoculation have been characterized using RNA-Seq technology. More than 100 million sequence reads were generated for condition (inoculated/uninoculated). The sequence reads were analyzed to measure gene expression levels, to detect alternative splicing events and single nucleotide polymorphisms. We observed 2,296 and 2,578 differentially expressed genes (DEGs) 72 hours after inoculation for the resistant and susceptible genotypes, respectively, of which 1018 were in common and showed 5,342 SNPs variants. About 320,000 and 175,000 reads mapped on Fusarium genome in the susceptible and resistant genotypes, respectively, and 129 fungal genes were differentially regulated in both lines. The identification of plant DEGs that interact with fungus will produce useful tools for the identification of candidate genes, the development of molecular markers and their use for selection of resistant maize genotypes by means of marker assisted selection.
Lingua originaleEnglish
Titolo della pubblicazione ospiteFIFTH SIBV CONGRESS towards EXPO 2015
Pagine59
Numero di pagine1
Stato di pubblicazionePubblicato - 2013
EventoFIFTH SIBV CONGRESS - Foggia
Durata: 18 set 201320 set 2013

Convegno

ConvegnoFIFTH SIBV CONGRESS
CittàFoggia
Periodo18/9/1320/9/13

Keywords

  • RNA-Seq, Fusarium ear rot, candidate genes, Zea mays

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