[Analysis of point mutations of Ha-ras gene in basaliomas and spinaliomas usiang the polymerase chain reaction (PCR) technic]

Ketty Peris, L Cerroni, S Chimenti, Hp Soyer, H Kerl, H. Höfler

Risultato della ricerca: Contributo in rivistaArticolo in rivista

Abstract

Polymerase chain reaction is a recently developed procedure to detect point mutations in human genomic DNA. The sensitivity and specificity of this technique which requires a very little amount of DNA, have allowed the large scale screening of tumors. The Authors report the detection of point mutations of Ha-ras gene at codon 12 in 12 basal cell carcinomas and 10 cutaneous squamous cell carcinomas by the use of polymerase chain reaction in paraffin-embedded tissue sections. The gene alteration was identified in 2 basaliomas whereas no squamous cell carcinoma showed point mutations. These results suggest that point mutations of Ha-ras gene do not represent a general activating mechanism in cutaneous epitheliomas.
Titolo tradotto del contributo[Autom. eng. transl.] [Analysis of point mutations of Ha-ras gene in basaliomas and spinaliomas usiang the polymerase chain reaction (PCR) technic]
Lingua originaleItalian
pagine (da-a)429-434
Numero di pagine6
RivistaGIORNALE ITALIANO DI DERMATOLOGIA E VENEREOLOGIA
Volume124
Stato di pubblicazionePubblicato - 1989

Keywords

  • Carcinoma, Basal Cell
  • Carcinoma, Squamous Cell
  • Codon
  • Humans
  • Mutation
  • Polymerase Chain Reaction
  • Skin Neoplasms

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