TY - JOUR
T1 - Analysis of immune reconstitution in children undergoing cord blood transplantation
AU - Moretta, Antonia
AU - Maccario, Rita
AU - Fagioli, Franca
AU - Giraldi, Eugenia
AU - Busca, Alessandro
AU - Montagna, Daniela
AU - Miniero, Roberto
AU - Comoli, Patrizia
AU - Giorgiani, Giovanna
AU - Zecca, Marco
AU - Pagani, Sara
AU - Locatelli, Franco
PY - 2001
Y1 - 2001
N2 - Objective. The aim of this study was to investigate and compare immune reconstitution in allogeneic cord blood transplantation (CBT) and bone marrow transplantation (BMT) recipients.Patients and Methods. Twenty-three children underwent CBT from either human leukocyte antigen-identical siblings (11 cases) or unrelated donors (12 cases) were enrolled in the study, together with 23 matched children receiving BMT. Patients were analyzed 2-3 and 12-15 months after transplant. Recovery of T-, B-, and NK-lymphocyte subsets, proliferative in vitro response to mitogens, as well as cytotoxic activities, were investigated.Results. CBT recipients showed a marked increase in the number of B lymphocytes as compared with patients who underwent BMT (p < 0.001). The absolute number of CD3(+) and CD8(+) T cells, as well as the proliferative response to T-cell mitogens, recovered with time after transplantation, irrespective of the source of stem cells used. Recipients of unrelated CBT had a better recovery of CD4(+) T lymphocytes (p < 0.01). Among patients experiencing acute graft-versus-host disease (GVHD), children given CBT had a much greater production of CD4(+) CD45RA(+) T cells than BMT recipients (p < 0.005). Recovery of NK cell number end innate cytotoxic activities was fast, irrespective of the source of stem cells used.Conclusions. Despite the much lower number of lymphocytes transferred with the graft, recovery of lymphocyte number and function toward normal in CBT recipients was rapid and comparable to that observed after transplantation of bone marrow progenitors. This prompt immune recovery possibly was favored by the reduced incidence and severity of GVHD observed in children who underwent CBT. (C) 2001 International Society for Experimental Hematology. Published by Elsevier Science Inc.
AB - Objective. The aim of this study was to investigate and compare immune reconstitution in allogeneic cord blood transplantation (CBT) and bone marrow transplantation (BMT) recipients.Patients and Methods. Twenty-three children underwent CBT from either human leukocyte antigen-identical siblings (11 cases) or unrelated donors (12 cases) were enrolled in the study, together with 23 matched children receiving BMT. Patients were analyzed 2-3 and 12-15 months after transplant. Recovery of T-, B-, and NK-lymphocyte subsets, proliferative in vitro response to mitogens, as well as cytotoxic activities, were investigated.Results. CBT recipients showed a marked increase in the number of B lymphocytes as compared with patients who underwent BMT (p < 0.001). The absolute number of CD3(+) and CD8(+) T cells, as well as the proliferative response to T-cell mitogens, recovered with time after transplantation, irrespective of the source of stem cells used. Recipients of unrelated CBT had a better recovery of CD4(+) T lymphocytes (p < 0.01). Among patients experiencing acute graft-versus-host disease (GVHD), children given CBT had a much greater production of CD4(+) CD45RA(+) T cells than BMT recipients (p < 0.005). Recovery of NK cell number end innate cytotoxic activities was fast, irrespective of the source of stem cells used.Conclusions. Despite the much lower number of lymphocytes transferred with the graft, recovery of lymphocyte number and function toward normal in CBT recipients was rapid and comparable to that observed after transplantation of bone marrow progenitors. This prompt immune recovery possibly was favored by the reduced incidence and severity of GVHD observed in children who underwent CBT. (C) 2001 International Society for Experimental Hematology. Published by Elsevier Science Inc.
KW - Bone Marrow Transplantation
KW - Bone Marrow Transplantation
UR - http://hdl.handle.net/10807/262664
U2 - 10.1016/s0301-472x(00)00667-6
DO - 10.1016/s0301-472x(00)00667-6
M3 - Article
SN - 0301-472X
VL - 29
SP - 371
EP - 379
JO - Experimental Hematology
JF - Experimental Hematology
ER -