Analysis of conjugated bile acids by high performance liquid chromatography and mass spectrometry

Geltrude Mingrone, Av Greco, L Boniforti, S. Passi

Risultato della ricerca: Contributo in rivistaArticolo in rivista

11 Citazioni (Scopus)

Abstract

Because of the known advantages of coupling high performance liquid chromatography with mass spectrometry (HPLC-MS) in biological fluids, studies on the reversed-phase HPLC-MS system for direct analysis of conjugated bile acids in human bile samples are described. Ten samples of gallbladder bile of apparently healthy subjects were examined. The amounts of each tauro- and glycoconjugated bile acid as trifluoracetate were determined by mass fragmentography. Quantitation of at least 1 ng of each bile acid was possible.
Lingua originaleEnglish
pagine (da-a)90-95
Numero di pagine6
RivistaLipids
Volume18
Stato di pubblicazionePubblicato - 1983

Keywords

  • Animals
  • Bile Acids and Salts
  • Chromatography, High Pressure Liquid
  • Gas Chromatography-Mass Spectrometry
  • Structure-Activity Relationship

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