Because of the known advantages of coupling high performance liquid chromatography with mass spectrometry (HPLC-MS) in biological fluids, studies on the reversed-phase HPLC-MS system for direct analysis of conjugated bile acids in human bile samples are described. Ten samples of gallbladder bile of apparently healthy subjects were examined. The amounts of each tauro- and glycoconjugated bile acid as trifluoracetate were determined by mass fragmentography. Quantitation of at least 1 ng of each bile acid was possible.
|Numero di pagine||6|
|Stato di pubblicazione||Pubblicato - 1983|
- Bile Acids and Salts
- Chromatography, High Pressure Liquid
- Gas Chromatography-Mass Spectrometry
- Structure-Activity Relationship