Analysis of anabolic steroids in hair by GC/MS/MS

Riccardo Rossi, C Gambelunghe, M Sommavilla, C Ferranti, K Aroni, N Manes, M. Bacci

Risultato della ricerca: Contributo in rivistaArticolo in rivista

30 Citazioni (Scopus)

Abstract

A simple and sensitive gas chromatography/tandem mass spectrometry (GC/MS/MS) method is described for the detection of anabolic steroids, usually found in keratin matrix at very low concentrations. Hair samples from seven athletes who spontaneously reported their abuse of anabolic steroids, and in a single case cocaine, were analyzed for methyltestosterone, nandrolone, boldenone, fluoxymesterolone, cocaine and its metabolite benzoylecgonine. Anabolic steroids were determinate by digestion of hair samples in 1 m NaOH for 15 min at 95 degrees C. After cooling, samples were purificated by solid-phase and liquid-liquid extraction, then anabolic steroids were converted to their trimethylsilyl derivative and finally analyzed by GC/MS/MS. For detection of cocaine and benzoylecgonine, hair samples were extracted with methanol in an ultrasonic bath for 2 h at 56 degrees C then overnight in a thermostatic bath at the same temperature. After the incubation, methanol was evaporated to dryness, and benzoylecgonine was converted to its trimethylsilyl derivative prior of GC/MS/MS analysis. Results obtained are in agreement with the athletes' reports, confirming that hair is a valid biological matrix to establish long-term intake of drugs.
Lingua originaleEnglish
pagine (da-a)369-375
Numero di pagine7
RivistaBiomedical Chromatography
Volume21
DOI
Stato di pubblicazionePubblicato - 2007

Keywords

  • Anabolic Agents
  • Gas Chromatography-Mass Spectrometry
  • Hair
  • Humans
  • Reference Standards
  • Sports
  • Steroids
  • Substance Abuse Detection
  • Tandem Mass Spectrometry

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