Abstract
A method for isolating potential probiotic lactobacilli
directly fromtraditional milk-based foods was developed.
The novel digestion/enrichment protocol was set up taking
care to minimize the protective effect of milk proteins and fats
and was validated testing three commercial fermented milks
containing well-known probiotic Lactobacillus strains. Only
probiotic bacteria claimed in the label were isolated from
two out of three commercial fermented milks. The application
of the new protocol to 15 raw milk samples and 6
traditional fermented milk samples made it feasible to isolate
11 potential probiotic Lactobacillus strains belonging to
Lactobacillus brevis, Lactobacillus fermentum, Lactobacillus
gasseri, Lactobacillus johnsonii, Lactobacillus plantarum,
Lactobacillus reuteri, and Lactobacillus vaginalis species.
Even though further analyses need to ascertain functional
properties of these lactobacilli, the novel protocol set-up
makes it feasible to isolate quickly potential probiotic strains
from traditional milk-based foods reducing the amount of
time required by traditional procedures that, in addition, do
not allow to isolate microorganisms occurring as subdominant
populations.
Lingua originale | English |
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pagine (da-a) | 331-342 |
Numero di pagine | 12 |
Rivista | Applied Microbiology and Biotechnology |
Volume | volume numero 90 |
Stato di pubblicazione | Pubblicato - 2011 |
Keywords
- Bile tolerance
- Complex microflora
- Lactobacillus acidophilus species group
- Probiotic bacteria