Administration of PLP139-151primes T cells distinct from those spontaneously responsive in vitro to this antigen

Gabriele Di Sante, Francesco Ria, Romina Penitente, Chiara Nicolo', Peter Van Den Elzen, Chiara Agrati, Francesca Aloisi, Eli E. Sercarz

Risultato della ricerca: Contributo in rivistaArticolo in rivista

12 Citazioni (Scopus)

Abstract

We examined the TCR repertoire used by naive SJL mice in their in vitro spontaneous response to proteolipid protein (PLP) 139-151 by Vβ-Jβ spectratyping and compared it to that used after immunization with the peptide. T cells from immunized mice use the public rearrangement Vβ 10-Jβ 1.1, but naive mice do not; in contrast, TCR CDR3-β rearrangements of Vβ 18-Jβ 1.2 and Vβ 9-Jβ l.2 consistently are associated with the spontaneous response. T cells involved in spontaneous and induced responses can each recognize PLPI39-I51presented in vivo, but its s.c. administration has different consequences for the two repertoires. Four days after immunization, T cells associated with spontaneous responsiveness appear in the draining lymph nodes but disappear by day 10 and never appear elsewhere. Simultaneously, Vβ 10-Jβ 1.1 T cells are likewise activated in the lymph nodes by day 4 and spread to the spleen by day 10. Eight- to 10-wk-old naive mice use a narrower repertoire of TCRs than do immunized age-matched mice. Induced Vβ 10-Jβ 1.1 T cells home to the CNS during experimental autoimmune encephalomyelitis, whereas we failed to detect Vβ 18-Jβ 1.2 and Vβ 19-Jβ 1.2 TCR rearrangements in the CNS. Thus, we observe that administration of PLP139-151primes a T cell repertoire distinct from the one responsible for spontaneous responsiveness. This "immunized" repertoire substitutes for the naive one and becomes dominant at the time of disease onset. Copyright © 2008 by The American Association of Immunologists, Inc.
Lingua originaleEnglish
pagine (da-a)6611-6622
Numero di pagine12
RivistaJOURNAL OF IMMUNOLOGY
Volume180
DOI
Stato di pubblicazionePubblicato - 2008

Keywords

  • Immunology

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