ace, Which encodes an adhesin in Enterococcus faecalis, is regulated by Ers and is involved in virulence

Francois Lebreton, Eliette Riboulet-Bisson, Pascale Serror, Maurizio Sanguinetti, Brunella Posteraro, Riccardo Torelli, Axel Hartke, Yanick Auffray, Jean-Christophe Giard

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

80 Citazioni (Scopus)

Abstract

Enterococcus faecalis is an opportunistic pathogen that causes numerous infectious diseases in humans and is a major agent of nosocomial infections. In this work, we showed that the recently identified transcriptional regulator Ers (PrfA like), known to be involved in the cellular metabolism and the virulence of E. faecalis, acts as a repressor of ace, which encodes a collagen-binding protein. We characterized the promoter region of ace, and transcriptional analysis by reverse transcription-quantitative PCR and mobility shift protein-DNA binding assays revealed that Ers directly regulates the expression of ace. Transcription of ace appeared to be induced by the presence of bile salts, probably via the deregulation of ers. Moreover, with an ace deletion mutant and the complemented strain and by using an insect (Galleria mellonella) virulence model, as well as in vivo-in vitro murine macrophage models, we demonstrated for the first time that Ace can be considered a virulence factor for E. faecalis. Furthermore, animal experiments revealed that Ace is also involved in urinary tract infection by E. faecalis.
Lingua originaleEnglish
pagine (da-a)2832-2839
Numero di pagine8
RivistaInfection and Immunity
Volume77
DOI
Stato di pubblicazionePubblicato - 2009

Keywords

  • enterococcus

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