Ability of polyurethane foams to support placenta-derived cell adhesion and osteogenic differentiation: preliminary results

Ornella Parolini, Daniele Rossi, S. Bertoldi, S. Farè, M. Denegri, D. Rossi, H. J. Haugen, M. C. Tanzi

Risultato della ricerca: Contributo in rivistaArticolo in rivista

28 Citazioni (Scopus)

Abstract

In bone tissue reconstruction, the use of engineered constructs created by mesenchymal stem cells (MSCs) that differentiate and proliferate into 3D porous scaffolds is an appealing alternative to clinical therapies. Human placenta represents a possible source of MSCs, as it is readily available without invasive procedures and because of the phenotypic plasticity of many of the cell types isolated from this tissue. The scaffold considered in this work is a slowly degradable polyurethane foam (EF PU foam), synthesized and characterized for morphology and in vitro interaction with chorion mesenchymal cells (CMCs). These cells were isolated from human term placenta and cultured onto the EF PU foam using two different culture media (EMEM and NH osteogenic differentiation medium). Synthesized EF PU foam showed homogeneous pore size and distribution, with 89% open porosity. In vitro tests showed CMCs scaffold colonization, as confirmed by Scanning Electron Microscopy (SEM) observations and hematoxylin-eosin staining. Alizarin Red staining revealed the presence of a small amount of calcium deposition for the samples treated with the osteogenic differentiation medium. Therefore, the proposed EF PU foam appears to stimulate cell adhesion in vitro, sustaining CMCs growth and differentiation into the osteogenic lineage.
Lingua originaleEnglish
pagine (da-a)1005-1011
Numero di pagine7
RivistaJOURNAL OF MATERIALS SCIENCE. MATERIALS IN MEDICINE
Volume21
DOI
Stato di pubblicazionePubblicato - 2010

Keywords

  • Bone Transplantation
  • Cell Adhesion
  • Cell Differentiation
  • Chorion
  • Culture Media
  • Female
  • Humans
  • Imaging, Three-Dimensional
  • Mesoderm
  • Microscopy, Electron, Scanning
  • Osteogenesis
  • Placenta
  • Polyurethanes
  • Pregnancy
  • Tomography, X-Ray Computed

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