A small-molecule targeting the microRNA binding domain of argonaute 2 improves the retinoic acid differentiation response of the acute promyelocytic leukemia cell line NB4

Silvia Masciarelli; Roberto Quaranta; Ilaria Iosue; Gianni Colotti; Fabrizio Padula; Greta Varchi; Francesco Fazi; Alberto Del Rio

doi:10.1021/cb500286b

A small-molecule targeting the microRNA binding domain of argonaute 2 improves the retinoic acid differentiation response of the acute promyelocytic leukemia cell line NB4

Silvia Masciarelli
, Roberto Quaranta
, Ilaria Iosue
, Gianni Colotti
, Fabrizio Padula
, Greta Varchi
, Francesco Fazi
, Alberto Del Rio

Università Cattolica del Sacro Cuore

University of Rome La Sapienza
National Research Council of Italy

Risultato della ricerca: Contributo in rivista › Articolo

23 Citazioni (Scopus)

Abstract

Argonaute proteins are pivotal regulators of gene expression mediating miRNAs function. Modulating their activity would be extremely useful to elucidate the processes governing small-RNAs-guided gene silencing. We report the identification of a chemical compound able to compete with Argonaute 2 miRNAs binding, and we demonstrate that this functional inhibition determines effects similar to Argonaute 2 shRNA-mediated down-regulation, favoring granulocytic differentiation of the acute promyelocytic leukemia cell line NB4 in response to retinoic acid. © 2014 American Chemical Society.

Lingua originale	Inglese
pagine (da-a)	1674-1679
Numero di pagine	6
Rivista	ACS Chemical Biology
Volume	9
DOI	https://doi.org/10.1021/cb500286b
Stato di pubblicazione	Pubblicato - 2014

Keywords

Argonaute Proteins
Cell Differentiation
Cell Line, Tumor
Humans
Leukemia, Promyelocytic, Acute
MicroRNAs
Small Molecule Libraries
Tretinoin

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10.1021/cb500286b

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title = "A small-molecule targeting the microRNA binding domain of argonaute 2 improves the retinoic acid differentiation response of the acute promyelocytic leukemia cell line NB4",

abstract = "Argonaute proteins are pivotal regulators of gene expression mediating miRNAs function. Modulating their activity would be extremely useful to elucidate the processes governing small-RNAs-guided gene silencing. We report the identification of a chemical compound able to compete with Argonaute 2 miRNAs binding, and we demonstrate that this functional inhibition determines effects similar to Argonaute 2 shRNA-mediated down-regulation, favoring granulocytic differentiation of the acute promyelocytic leukemia cell line NB4 in response to retinoic acid. {\textcopyright} 2014 American Chemical Society.",

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doi = "10.1021/cb500286b",

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journal = "ACS Chemical Biology",

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T1 - A small-molecule targeting the microRNA binding domain of argonaute 2 improves the retinoic acid differentiation response of the acute promyelocytic leukemia cell line NB4

AU - Masciarelli, Silvia

AU - Quaranta, Roberto

AU - Iosue, Ilaria

AU - Colotti, Gianni

AU - Padula, Fabrizio

AU - Varchi, Greta

AU - Fazi, Francesco

AU - Del Rio, Alberto

PY - 2014

Y1 - 2014

N2 - Argonaute proteins are pivotal regulators of gene expression mediating miRNAs function. Modulating their activity would be extremely useful to elucidate the processes governing small-RNAs-guided gene silencing. We report the identification of a chemical compound able to compete with Argonaute 2 miRNAs binding, and we demonstrate that this functional inhibition determines effects similar to Argonaute 2 shRNA-mediated down-regulation, favoring granulocytic differentiation of the acute promyelocytic leukemia cell line NB4 in response to retinoic acid. © 2014 American Chemical Society.

AB - Argonaute proteins are pivotal regulators of gene expression mediating miRNAs function. Modulating their activity would be extremely useful to elucidate the processes governing small-RNAs-guided gene silencing. We report the identification of a chemical compound able to compete with Argonaute 2 miRNAs binding, and we demonstrate that this functional inhibition determines effects similar to Argonaute 2 shRNA-mediated down-regulation, favoring granulocytic differentiation of the acute promyelocytic leukemia cell line NB4 in response to retinoic acid. © 2014 American Chemical Society.

KW - Argonaute Proteins

KW - Cell Differentiation

KW - Cell Line, Tumor

KW - Humans

KW - Leukemia, Promyelocytic, Acute

KW - MicroRNAs

KW - Small Molecule Libraries

KW - Tretinoin

KW - Argonaute Proteins

KW - Cell Differentiation

KW - Cell Line, Tumor

KW - Humans

KW - Leukemia, Promyelocytic, Acute

KW - MicroRNAs

KW - Small Molecule Libraries

KW - Tretinoin

UR - http://hdl.handle.net/10807/147544

UR - http://pubs.acs.org/journal/acbcct

U2 - 10.1021/cb500286b

DO - 10.1021/cb500286b

M3 - Article

SN - 1554-8929

VL - 9

SP - 1674

EP - 1679

JO - ACS Chemical Biology

JF - ACS Chemical Biology

ER -

A small-molecule targeting the microRNA binding domain of argonaute 2 improves the retinoic acid differentiation response of the acute promyelocytic leukemia cell line NB4

Abstract

Keywords

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